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Tissue-engineered blood vessel cultured in vitro

A technology of blood vessels and tissue cells, which can be applied to the devices of blood vessels and human tubular structures, etc., can solve the problems of unsatisfactory effects, source and strength limitations, etc.

Inactive Publication Date: 2013-04-03
TIANJIN SANNIE BIOENG TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Autovascular is widely used clinically, but due to the limitation of source and intensity, satisfactory results cannot be achieved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Take 0.1 g of commercially available type I collagen and soak it in 100 ml of 0.2 mol / L acetic acid solution. Stir well to fully dissolve, then place in the glass tube of a tumble mixer and roll the coating. Pour off excess collagen solution after forming a uniform collagen film. Pour 0.1 g of commercially available polyethylene glycol into 100 ml of sterilized physiological saline and stir evenly, take an appropriate amount of polyethylene glycol liquid and drop it on the collagen film, and roll the coating with a drum agitator. Press 1.25×10 5 Add the recipient's own bone marrow-differentiated smooth muscle cells at a concentration of 1 / ml, plant continuously for 2 days, and then simultaneously press 1.25×10 5 Individual / ml concentration planted recipient's own bone marrow differentiated fibroblasts. Planted continuously for 3 days respectively. Next, the recipient's own bone marrow vascular endothelial progenitor cells and vascular endothelial growth factor were ...

Embodiment 2

[0014] Take 0.2 g of commercially available type I collagen and soak it in 100 ml of 0.5 mol / L acetic acid solution. Stir well to fully dissolve, then place in the glass tube of a tumble mixer and roll the coating. Pour off excess collagen solution after forming a uniform collagen film. Pour 0.5 g of commercially available polyethylene glycol into 100 ml of sterilized physiological saline and stir evenly, take an appropriate amount of polyethylene glycol liquid and drop it on the collagen film, and roll the coating with a drum agitator. Press 1.25×10 5 Add the recipient's own bone marrow-differentiated smooth muscle cells at a concentration of 1 / ml, plant continuously for 2 days, and then simultaneously press 1.25×10 5 Individual / ml concentration planted recipient's own bone marrow differentiated fibroblasts. Planted continuously for 3 days respectively. Next, the recipient's own bone marrow vascular endothelial progenitor cells and vascular endothelial growth factor were ...

Embodiment 3

[0016] Take 1.0 g of commercially available type I collagen and soak it in 100 ml of 0.01 mol / L acetic acid solution. Stir well to fully dissolve, then place in the glass tube of a tumble mixer and roll the coating. Pour off excess collagen solution after forming a uniform collagen film. Pour 0.1 g of commercially available polyethylene glycol into 100 ml of sterilized physiological saline and stir evenly, take an appropriate amount of polyethylene glycol liquid and drop it on the collagen film, and roll the coating with a drum agitator. Press 1.25×10 5 Add the recipient's own bone marrow-differentiated smooth muscle cells at a concentration of 1 / ml, plant continuously for 2 days, and then simultaneously press 1.25×10 5 Individual / ml concentration planted recipient's own bone marrow differentiated fibroblasts. Planted continuously for 3 days respectively. Next, the recipient's own bone marrow vascular endothelial progenitor cells and vascular endothelial growth factor were...

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PUM

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Abstract

The invention relates to a vascular graft used for a clinical vascular grafting surgery and a preparation method therefore, which belongs to the field of medical biomaterials, and mainly solves the problems that clinical materials drawing from autologous vessels is limited at present, and the surgery is complex; the allograft vascular risk is high, polymer materials implanted in the body have poor complicance or are calcified and the like. According to the characteristic that natural vessels have different structures and different tissue cells, the receptor self tissue cells are planted for three layers, and finally the vascular graft is prepared by simulating mechanical parameters born by vessels in vivo and the blood flow condition, so that the purpose of autologous vascular repair is achieved. The material prepared by the method is formed by culturing I type collagen and the receptor self tissue cells in a vascular bioreactor, is close to the autologous vessels, has good biocompatibility, growth potential and stronger mechanical property, is not easily infected, can bear transmural pressure and the complicance force of blood flow to the vascular wall, has better roles of anti-thrombosis and anti-platelet adhesion, and is beneficial to vascular repair of the vascular grafting surgery.

Description

technical field [0001] The invention relates to a tissue engineering blood vessel produced by culturing type I collagen and tissue cells in a blood vessel bioreactor. It has strong mechanical properties, can withstand the transmural pressure and the compliance force of blood flow to the vessel wall, and has good anti-thrombosis and anti-platelet adhesion effects. Good biocompatibility. Vascular grafts for cardiovascular and cerebrovascular diseases. It belongs to the field of medical biomaterials. Background technique [0002] Arterial ischemic disease represented by atherosclerotic heart disease is one of the main causes of human death. The main treatment is arterial grafting. In addition, congenital heart disease is a common congenital malformation that requires surgical correction, and some complicated congenital heart disease operations also require the application of vascular grafts. In the past, most of the vascular grafts we used were polymer artificial materials...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61F2/06
Inventor 万力张春红
Owner TIANJIN SANNIE BIOENG TECH
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