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Method for increasing lipolysis using a composition comprising bioactive nelumbo nucifera (LOTUS) extract

A kind of fat decomposition, lotus extract technology, applied in the field of beauty

Active Publication Date: 2019-04-26
ISP INVESTMENTS INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] However, these products often have moderate or limited efficacy over time

Method used

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  • Method for increasing lipolysis using a composition comprising bioactive nelumbo nucifera (LOTUS) extract
  • Method for increasing lipolysis using a composition comprising bioactive nelumbo nucifera (LOTUS) extract
  • Method for increasing lipolysis using a composition comprising bioactive nelumbo nucifera (LOTUS) extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0121] Example 1: Analysis of Bioactive Lotus Extract Fractions (Cytoplasmic / Cytosolic Fraction or Cellular Essence Fraction)

[0122] The bioactive components from the lotus plant were obtained according to the Zeta fraction technology method described in U.S. Patent Nos. 7,442,391; 7,473,435; 7,537,791; 8,043,635; The manufacturing method employs grinding and squeezing fresh live plants to obtain plant cell juice (intracellular colloidal dispersion) and treating it with electromagnetic waves at a frequency effective to cause separation of the membrane fraction from the cell juice to produce the cell cytoplasmic / cytosolic grade Fraction, which is substantially free of membrane fraction.

[0123] The cytoplasmic / cytosolic fraction is further processed under conditions effective to separate the cytoplasmic / cytosolic fraction into its component parts, such as a cytoplasmic fraction and a cytosolic fraction. The cytosolic fraction mainly contains low molecular weight component...

Embodiment 2

[0139] Example 2: Evaluation of lotus (Nelumbo nucifera) extract on lipolysis

[0140] This study aimed to evaluate the in vitro effect of lotus (Nelumbo nucifera) extract on lipolysis by assessing the release of glycerol on preadipocytes. Lipolysis is the hydrolysis of triglycerides into glycerol and fatty acids. The amount of glycerol released will be proportional to the amount of stored triglycerides and the degree of lipolysis

[0141] Protocol: 3T3-L1 preadipocytes (ATCC) were cultured in DMEM 4.5 g / L glucose (Lonza) supplemented with 10% FBS (Lonza), 2 mM L-glutamine (Lonza) and 100 μg / ml Primocin (InvivoGen) .

[0142] Two days after cell confluence, differentiation into adipocytes was induced by adding 0.5 mM IBMX, 1 μM dexamethasone and 10 μg / ml insulin (Sigma) to the medium for 3 days. Then, IBMX and dexamethasone were removed, and insulin only was maintained for 3 to 4 days; then, insulin was removed, and cells were maintained in culture medium for another 3 da...

Embodiment 3

[0148] Example 3: In vivo study on the effect of lotus (Nelumbo nucifera) extract on weight loss

[0149] Protocol: Twenty healthy female volunteers aged 23 to 48 participated in this comparative double-blind study. Volunteers applied cream formula #TCAAG06 containing 0.5% lotus (Nelumbo nucifera) extract of Example 1 on one thigh and placebo on the other twice a day (morning and evening) for 8 weeks. The results of the Nelumbo nucifera extract effect were determined by centimeter measurements of the upper and lower thighs (circumference), temperature assessments of the upper thighs, and visual assessments by trained experts and volunteers. Depending on whether the data follow a normal distribution, a Student's t-test (one-tailed) or a Wilcoxon signed rank test (one-tailed) was used. Control visits have been done at D0, D28 and D56. For statistical analysis, a Student's t-test (one-tailed) or a Wilcoxon signed-rank test (one-tailed) was used depending on whether the data f...

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Abstract

The present invention is related to a cosmetic method for promoting lipolysis, slimming, draining, smooth skin surface, reducing the volume of localized fat deposits, the method including applying a topical composition comprising a Nelumbo nucijera (Lotus) Extract.

Description

technical field [0001] The present invention relates to the field of cosmetics, and more particularly to the field of cosmetic slimming methods. It concerns the cosmetic use of bioactive ingredients obtained from fresh (live) plants of the lotus (Nelumbo nucifera Gaertn) as a slimming and draining cosmetic agent. [0002] The present invention also relates to a method of cosmetic care comprising topical application on at least a part of the skin of the body or face of lotus (Nelumbo nucifera) extract in a composition containing a physiologically acceptable medium in order to obtain Weight loss, and more specifically the reduction of localized excess body fat. Background technique [0003] Subcutaneous fatty tissue is located under the skin. It is a connective tissue in which adipocytes predominate, organized in lobules approximately 5 mm in diameter, separated by thin connective bridges. Each adipocyte contains a large number of lipid vacuoles, basically containing trigly...

Claims

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Application Information

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IPC IPC(8): A61K8/97A61Q19/06A61Q90/00
CPCA61Q19/06A61Q90/00A61K8/9789A61K8/97A61Q19/00
Inventor K·屈居梅尔I·安贝尔N·东洛热O·杜埃娃-科加诺夫M·科加诺夫
Owner ISP INVESTMENTS INC
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