Anti-salt liquid fertilizer and preparation method thereof
A liquid and salt solution technology, applied in the field of salt-resistant liquid fertilizer and its preparation, can solve the problems of large amount of organic fertilizer and bacterial fertilizer, unstable raw materials, and high cost
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[0013] The invention provides a method for preparing a salt-resistant liquid fertilizer, the method steps comprising:
[0014] S10, respectively screening and culturing Bacillus subtilis and Bacillus amyloliquefaciens with a medium containing 0.4% to 0.8% of the mass fraction of salt, and obtaining salt-resistant Bacillus subtilis and salt-resistant starch that can survive in the medium containing salt Bacillus;
[0015] S20, further expand the resistant Bacillus subtilis and the resistant Bacillus amyloliquefaciens obtained in step S10, and collect and expand the cultured strains; The mass ratio of 30 to 50 is mixed into a mixed strain for later use;
[0016] S30, 12-14 parts of urea or urea ammonium nitrate solution, 4-5 parts of potassium dihydrogen phosphate, 4-6 parts of ammonium polyphosphate, 5-7 parts of potassium sulfate, 15-17 parts of potassium nitrate, Dissolve 36-52.5 parts of water in the dissolving tank to obtain a large amount of element solution;
[0017] S...
Embodiment 1
[0026] In this Example 1, the salt-resistant liquid fertilizer was prepared according to the following steps:
[0027] S11, the purchased agricultural Bacillus subtilis is inoculated with the Bacillus subtilis common culture medium (1L distilled water+20g glucose+15g peptone+5g sodium chloride+0.5g beef extract+20g agar) with 0.5wt% sodium carbonate, and the screening ability The bacterial strain of normal growth; And the bacterial strain that can normal growth continues to carry out mass culture again with the common substratum of Bacillus subtilis of 0.5wt% sodium carbonate;
[0028] S12, the purchased agricultural Bacillus amyloliquefaciens is inoculated and cultivated with the Bacillus amyloliquefaciens CM3 medium that adds 0.5wt% sodium carbonate, and the strains that can grow normally are screened; and the bacterial strains that can grow normally are continued to use 0.5wt% sodium carbonate The Bacillus amyloliquefaciens CM3 culture medium carries out mass cultivation ag...
Embodiment 2
[0033] In this embodiment 2, salt-resistant liquid fertilizer was prepared according to the following steps:
[0034] S11, the purchased agricultural Bacillus subtilis is inoculated with the Bacillus subtilis common culture medium (1L distilled water+20g glucose+15g peptone+5g sodium chloride+0.5g beef extract+20g agar) that has 0.4wt% sodium carbonate, and the screening ability The bacterial strain of normal growth; And the bacterial strain that can normal growth continues to carry out mass culture again with the common substratum of subtilis of 0.4wt% sodium carbonate;
[0035] S12, the purchased agricultural bacillus amyloliquefaciens is inoculated and cultivated with the bacillus amyloliquefaciens CM3 culture medium that adds 0.4wt% sodium carbonate, and the strains that can grow normally are screened; and the bacterial strains that can grow normally are continued to use 0.4wt% sodium carbonate The Bacillus amyloliquefaciens CM3 culture medium carries out mass cultivation ...
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