Molecular marker related to generation of inguinal hernia/scrotal hernia in pigs and application thereof

A technique for inguinal and scrotal hernias, applied in the field of marker-assisted selection of pigs, can solve problems such as failure to find the exact cause

Active Publication Date: 2019-04-19
HUAZHONG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Inguinal / scrotal hernia, as a common surgical disease, has been studied for quite a long time. Researchers have also tried to explain its pathogenesis from different angles such as anatomy, unclosed processus vaginalis, and increased intra-abdominal pressure. But so far, the exact cause of its occurrence has not been found

Method used

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  • Molecular marker related to generation of inguinal hernia/scrotal hernia in pigs and application thereof
  • Molecular marker related to generation of inguinal hernia/scrotal hernia in pigs and application thereof
  • Molecular marker related to generation of inguinal hernia/scrotal hernia in pigs and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Obtaining Segment of Pig PRKCE Gene and Establishment of SNP Detection Method

[0023] The experiment selected large white pigs, landrace pigs and their hybrid pig breeds, and designed the following primer pairs according to the pig PRKCE genome sequence (accession number Gene ID: 100524932). The specific sequences are as follows:

[0024] Forward primer F: 5'-ACCCTCAACGCTCTGTCC-3'

[0025] Reverse primer R: 5'-TAGGCCGCTTAACCAAAA-3'

[0026] PCR amplification was carried out in Large White pig, Landrace pig and hybrid pig genomic DNA using the above primer pairs.

[0027] The PCR reaction system is shown in Table 1.

[0028] Table 1 PCR reaction system

[0029]

[0030] The PCR reaction conditions are shown in Table 2.

[0031] Table 2 PCR reaction conditions

[0032]

[0033] The resulting PCR product was purified and cloned for sequence determination, and the sequencing work was completed by Beijing Aoke Dingsheng Biotechnology Co., Ltd. After blast comparis...

Embodiment 2

[0036] Detection and verification of polymorphism distribution of genetic markers of the present invention in different pig herds

[0037] In order to determine whether the SNP in the tenth intron region of the porcine PRKCE gene is related to the difference in pig phenotype, French Large White pigs (109 heads), French Landrace pigs (53 heads), American Large White pigs (39 heads) and Hybrid pigs (66 heads) were used as test materials, and the samples and data were collected from Zhejiang Kaisheng Ecological Agriculture Development Co., Ltd. Using conventional Taq α I-RFLP method was used for polymorphism detection, and the correlation between different genotypes of the SNP loci and pig inguinal scrotal hernia traits was analyzed. Independence test based on genotype frequency and gene frequency (X 2 ).

[0038] Table 3 PRKCE gene Taq α Comparison of the distribution of I-RFLP genotype frequency and allele frequency in patients with inguinal / scrotal hernia and normal indivi...

Embodiment 3

[0043] Application of rs328681942 site in large white pig breeding:

[0044] 50 American Large White pigs with inguinal hernia positive and 50 American Large White pigs with inguinal hernia negative in a pig farm were tested for molecular markers, using 5'-ACCCTCAACGCTCTGTCC-3' and R:5'-TAGGCCGCTTAACCAAAA-3 'Amplify the DNA and carry out restriction endonuclease Taq α I digestion, the results showed that a total of 50 samples of CC genotype were detected, all of which were diseased pigs, further confirming that the CC locus can be used as a potential genetic marker of inguinal scrotal hernia traits in Large White pigs.

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Abstract

The invention belongs to the technical field of genetic marker preparation of pigs, and specifically discloses a molecular marker related to pig inguinal hernia/scrotal hernia traits and an application thereof. The marker is screened from the porcine PRKCE gene, a C/T base mutation is generated at 230 base of a sequence shown in SEQ ID NO. 1, TaqalphaI-RFLP polymorphism is generated, the SNP siteis used to design primers, the correlation between the SNP marker and the inguinal hernia/scrotal hernia is analyzed, and the SNP mutation site in a tenth intron region of the porcine PRKCE gene is found to be significantly associated with the pig inguinal hernia/scrotal hernia traits. The molecular marker provides the novel marker resources for pig marker assisted selection.

Description

technical field [0001] The invention belongs to the technical field of marker-assisted selection of pigs, and specifically relates to a molecular marker related to the occurrence of pig inguinal scrotal hernia and its application. The molecular marker is isolated from the tenth intron region of the pig PRKCE gene. Background technique [0002] Inguinal scrotal hernia is a complex disease common in mammals, mainly manifested as abdominal viscera (gut or omentum) or ascites infiltrating into the groin or scrotum, causing local swelling and protrusion. Studies have shown that the occurrence of inguinal / scrotal hernia is the result of the joint action of genetic and environmental factors, and the heritability shows great variation due to different statistical populations, generally in the range of 0.2-0.6 (Gatphayak, et al. Present Situation of PorcineHemiainguinalis / scrotalis in Thailand. The Global Food & Product Chain—Dynamics, Innovations, Conficts, Strategies. Deutscher Tr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888
CPCC12Q1/6888C12Q2600/136C12Q2600/156C12Q2600/124
Inventor 郑嵘邹丹阳荣雷左波王国水毛慧敏
Owner HUAZHONG AGRI UNIV
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