Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of lignocellulose degrading strain protoplast

A technology of lignocellulose and protoplasts, applied in the field of protoplast preparation, can solve the problems of low activity of degrading strains and inability to use large-scale production

Inactive Publication Date: 2019-04-16
JILIN AGRI SCI & TECH COLLEGE
View PDF5 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the degrading strains screened so far have low activity and cannot be used for large-scale production. Therefore, mutagenesis breeding of filamentous fungi has become the main direction of research on lignocellulose degrading bacteria.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of lignocellulose degrading strain protoplast
  • Preparation method of lignocellulose degrading strain protoplast
  • Preparation method of lignocellulose degrading strain protoplast

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0022] Material

[0023] (1) Test strains

[0024] Ganoderma lucidum is isolated and preserved by the Microbiology Laboratory of the School of Bioengineering.

[0025] (2) Medium

[0026] Improved PDA medium: Add 20g of agar and water to 1000mL on the basis of the formula of the liquid strain activation medium.

[0027] Guaiacol screening medium: use a pipette to draw 0.04% guaiacol into the modified PDA medium.

[0028] Liquid strain activation medium: after peeling an appropriate amount of potatoes, accurately weigh 200.0 g, cut into small pieces, boil for 20 minutes, add magnesium sulfate 0.6g, glucose 20g, peptone 2g, potassium dihydrogen phosphate 0.5g, vitamin B1 0.05g, filter to take clarified potato juice, add water to make up to 1000mL.

[0029] Reagent

[0030] (1) Enzyme preparation

[0031] Lysozyme was purchased from Shanghai Yuanye Biotechnology Co., Ltd.

[0032] (2) Other reagents

[0033] Preparation of osmotic pressure stabilizer: accurately weigh KCl...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation method of a lignocellulose degrading strain protoplast. The preparation method comprises the following steps: after activating ganoderma lipsiense strains, inoculating a high-quality strain into a liquid strain activation medium for expansion culture, and selecting a 9-day mycelium to prepare the protoplast; conducting washing, sterile water washing and osmotic pressure stabilizer washing on the mycelium sequentially, adding 30 mL of mercaptoethanol for shock treatment, filtering after hyphae are broken, then washing with 0.7 mol / L of MgSO4 solution, centrifuging, taking a precipitate with a supernatant removed, removing moisture, placing the mycelium precipitate and 1.0% lywallzyme solution in the proportion of 1:10 by weight in a centrifuge tube, shaking evenly, hydrolyzing for 3.0 h and filtering. The precipitate obtained after centrifugation of the obtained filtrate is the protoplast of the mycelium of ganoderma lipsiense. The protoplast preparation rate is reaches high up to 9.15*106 cells / mL.

Description

technical field [0001] The invention relates to a method for preparing protoplasts, in particular to a method for preparing protoplasts of lignocellulose-degrading bacterial strains. Background technique [0002] The energy problem has always been a global problem. As a large amount of non-renewable energy is exploited and used by humans, the energy crisis has threatened human life and social stability. Excavating and researching renewable energy has become an important problem to be solved in the strategy of sustainable development. [0003] Lignocellulose is currently the most widely distributed and abundant renewable resource on the earth, and its dry weight accounts for 30% to 50% of the dry weight of plants in the world. At present, many substances containing a lot of cellulose are abandoned, such as the cellulose substances produced by plants during photosynthesis, the waste left by human beings due to social activities, and so on. The common ones in our country are ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 刘晓丹
Owner JILIN AGRI SCI & TECH COLLEGE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com