Ready-to-use culture apparatus for most probable number detection of flora

Abstract

The invention relates to a ready-to-use culture apparatus for the most probable number detection of a flora. A culture detection tube comprises a main pipe, a gas collection pipe and a pipe cap for sealing the main pipe. The main pipe is of a curved hose structure and is connected with the gas collection pipe. The main pipe, the gas collection pipe and the pipe cap form a sealed chamber, the length of the main pipe is greater than that of the gas collection pipe, and the inner diameter of the main pipe is greater than that of the gas collection pipe. The culture detection tube is the externalgas collection pipe. Compared with a built-in small inverted pipe which is disposed 180 degrees upside down in the prior art, the angle between the gas collection pipe and the main pipe can be flexibly set, which greatly reduces exhausting difficulty.

Description

technical field [0001] The disclosure belongs to the field of flora detection devices, and in particular relates to a ready-to-use culture device for detecting the most probable number of flora. Background technique [0002] The information disclosed in this Background section is only intended to increase the understanding of the general background of the disclosure, and is not necessarily to be taken as an acknowledgment or any form of suggestion that the information constitutes prior art that is already known to those skilled in the art. [0003] The detection of coliforms, heat-resistant coliforms (fecal coliforms) and Escherichia coli in the hygienic microbiological indicators of public products such as water, food, cosmetics and public places includes the multi-tube fermentation most probable number (MPN) method, Membrane filtration method, plate counting method, enzyme substrate method, etc. Among them, the multi-tube fermentation most probable number (MPN) method has...

AI Technical Summary

Problems solved by technology

Either the culture medium manufacturer manufactures according to the above steps, or after preparing the liquid culture medium and autoclaving it, it is divided into test tubes containing small inverted tubes (both are sterile and sterilized by ethylene oxide or radiation) and manufactured , or take an appropriate amount of sterile dry powder and subpackage it in a test tube containing a small inverted tube (both are sterile, sterilized by oxyethane or radiation); but the ready-to-use liquid medium prepared above During the transportation of the fermentation tube, it is easy to refill the small inverted tube with gas. Before using each fermentation tube, the experimenter needs to carefully invert it several times to let the small inverted tube discharge the gas. The small inverted tube has a small diameter and forms an angle of 180° with the fermentation test tube. In the inverted state, it is difficult to discharge the gas, and it is necessary to rely on skilled experimenters to select a suitable angle, slowly fill the liquid, and press out the gas; the ready-to-use fermentation tube containing the dry powder medium is easy to fill when the sample liquid is added to the small inverted tube In addition to the above-mentioned problems, there is also the limitation that the dry powder is difficult to dissolve, especially the medium in the small inverted tube is extremely difficult to dissolve, and each tube needs to be shaken repeatedly and stood still, so each tube must be handled carefully , it takes a long time

However, coliforms, heat-resistant coliforms (fecal coliforms) and Escherichia coli belong to the hygienic microbial indicators of public products such as water, food, cosmetics and public places. A sample requires 1-30 fermentation tubes, so no matter whether the experimenter prepares the medium himself or uses the ready-to-use fermentation tube provided by the medium manufacturer to discharge small inverted tube gas, it will consume a lot of human resources and time resources, and For the liquid type ready-to-use fermentation tube, the storage requirements are relatively high, and the shelf life is short, which increases the cost of use; once the dry powder-type ready-to-use fermentation tube is filled with more dry powder in the small inverted tube, it does not use the medium to mix in the sample liquid. When mixing, it is easy to generate fine bubbles in the small inverted tube, which increases the difficulty of exhausting, reduces the quality of detection and increases the cost of detection.

Therefore, the small inverted tube exhaust problem is a bottleneck technical limitation that affects the efficiency of the multi-tube fermentation most probable number (MPN) method, manpower and time costs, and the convenience of ready-to-use media.

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