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Method for collecting pig hypophysis and hypothalamus

A technique of hypothalamus and pituitary gland, applied in the field of collecting porcine pituitary gland and hypothalamus, can solve the problems of poor integrity of pituitary gland and hypothalamus, degradation of polluted RNA, mixed brain tissue, etc., and achieves simple method and good use effect. , the effect of simple and quick operation

Pending Publication Date: 2019-04-12
HENAN AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
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Problems solved by technology

Because the pituitary gland and hypothalamus obtained by this method are mechanically damaged, the integrity of the collected pituitary gland and hypothalamus is poor, and the brain tissue is easily mixed during the splitting process (such as brain, hypothalamus, cerebellum, etc.) , pituitary gland and other fragile tissues), it takes a long time to search for samples in half-cutting, and the samples are exposed to the air for a long time, resulting in serious pollution and serious RNA degradation. devastating effect

Method used

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  • Method for collecting pig hypophysis and hypothalamus
  • Method for collecting pig hypophysis and hypothalamus
  • Method for collecting pig hypophysis and hypothalamus

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Embodiment 1

[0025] The invention provides a method for collecting pig pituitary gland and hypothalamus,

[0026] A method for collecting pig pituitary gland and hypothalamus, comprising the steps:

[0027] 1) Take the head of a pig that has been slaughtered, and use a scalpel to cut open the skin with a scalpel at the middle position parallel to the direction of the nasal bone (this position is the symmetrical position of the left and right skulls, which itself has an incompletely healed line during development) bone suture, in which the skin is cut to facilitate the subsequent separation of the left and right skulls) the skin is peeled off to both sides to expose the skull, such as figure 1 shown.

[0028] 2) You can use scissors for small pigs, and use electric saws or hacksaws for large pigs to cut vertically and parallel to the direction of the nasal bone. Open the skull at an angle of 180°, cut into the bone by 0.2-0.4cm in a 20-day-old piglet, and the brain tissue in the head can ...

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Abstract

The invention discloses a method for collecting a pig hypophysis and hypothalamus. Crisscross cutting is adopted to enable subsequent skull to be completely split off without damaging brain tissue. According to the method for collecting the pig hypophysis and hypothalamus, the pig skull can be rapidly split off, a complete, low-polluted and low-degradative tissue sample of the hypophysis and the hypothalamus is obtained, and the collecting efficiency of a brain tissue sample is ensured; and the deficiencies that in the prior art, the polluted degree of the tissue such as the hypophysis and thehypothalamus is high, the tissue is prone to being lost and the like are overcome, the method is suitable for collection of large quantity of sample, and the operation is easy and rapid.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular, the invention relates to a method for collecting pig pituitary gland and hypothalamus. Background technique [0002] The pituitary gland is the most important endocrine gland in the body. It secretes a variety of hormones that play an important role in the body's metabolism, growth, development, and reproduction. The hypothalamus is the high-level center that regulates visceral activities under the cerebral cortex, regulating body temperature, food intake, and water balance. , blood sugar and endocrine gland activity and other important physiological functions; collecting intact and pollution-free pituitary gland and hypothalamus for total RNA extraction is of great significance to its follow-up research. Existing methods for collecting pig brain tissue samples, especially for pig pituitary gland and hypothalamus, mainly use electric saws or axes to split the skull longitudinally into ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/04G01N1/10
CPCG01N1/04G01N1/10
Inventor 李新建叶建伟李秀领王克君乔瑞敏韩雪蕾吕刚
Owner HENAN AGRICULTURAL UNIVERSITY
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