Elastic microfluidic chip for tuberculosis detection

Abstract

The invention discloses an elastic microfluidic chip for tuberculosis detection. The elastic microfluidic chip for tuberculosis detection comprises a lower base plate, a PDMS elastic reaction cavity and a top-layer chip module, wherein the top-layer chip module comprises a filter layer, a lower-layer double faced adhesive tape, a mixed layer, an upper-layer double faced adhesive tape, an upper closure piece and a rubber stopper. Plasma separation of a whole blood sample to be tested, simultaneous mixing of plasma and a sample diluent and volumetric quantification and detection of the diluted sample are executed. In the top-layer chip module, the diluted sample is driven to lateral flow fluorescent paper strip in a chip detection area to complete the immunochromatographic reaction, and singles of a test line and a control line on the fluorescent paper strip corresponding to the sample are read through simultaneous immunochromatographic reaction on a plurality of sample culture solutions, and therefore negative and positive judgement of a tuberculosis detecting result can be achieved. The elastic microfluidic chip has the advantages of small volume, simple structure, convenient operation and low cost, and lays a good foundation for achieving rapid on-site tuberculosis detection.

Description

technical field [0001] The invention relates to the fields of microfluidic technology and tuberculosis detection, in particular to an elastic microfluidic chip for tuberculosis detection and its application. Background technique [0002] Tuberculosis has always been the most important infectious disease threatening human health. For a long time, the diagnosis of tuberculosis has mainly been based on classic sputum smear acid-fast bacillus staining and chest X-ray examination. Due to the low positive rate of sputum smear examination and the low resolution of chest X-ray, early tuberculosis cases cannot be diagnosed . In particular, for early tuberculosis patients, X-rays are often difficult to accurately identify due to the small area of ​​tuberculosis infection. Therefore, the method to realize the accurate diagnosis of early tuberculosis has important clinical application value. The new tuberculosis detection method involves multiple steps such as sample cultivation, pre...

AI Technical Summary

Problems solved by technology

The new tuberculosis detection method involves multiple steps such as sample cultivation, pretreatment, reaction process, signal reading, negative and positive interpretation, etc. At the same time, it involves the use of multiple detection instruments. The reaction process is cumbersome, the operation is complicated, and the detection time is long. The high cost of detection is not conducive to the application and promotion of tuberculosis detection methods

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