Application of circ-PLEKHA1 molecular marker in blood to diagnosis of ESCC (esophageal squamous carcinoma)
A technology of circ-fam193a and molecular markers, applied in the field of tumor molecular biology, can solve the problems of large trauma and unacceptable patients
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[0031] 1. RNase R Digestion and Actinomycin-D Digestion
[0032] 2mg of total RNA was incubated at 37°C for 20 minutes with or without the addition of 3U / mg RNase R, followed by the use of the RNeasyMinElute kit. For actinomycin D treatment, 2 mg of total RNA was incubated at 37°C with or without adding 1 mg of actinomycin (actinomycin D, purchased from Sigma Reagent Company), and at 0, 6, 12, The resulting RNA was detected separately for 18 hours.
[0033] 2. Subjects
[0034] This study is based on the Department of Oncology, Nanjing Hospital Affiliated to Nanjing Medical University. The Department of Oncology treats patients with esophageal squamous cell carcinoma all the year round, and establishes a specimen bank, which can provide rich case data for this study. The tissues and blood of the study patients came from patients with esophageal squamous cell carcinoma who underwent radical or palliative surgical resection from 2013 to August 2017. The normal control blood s...
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