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Construction method of oxidative damage model for muscle cell of beef cattle and application of oxidative damage model

A technology for muscle cells and oxidative damage, applied in biochemical equipment and methods, animal cells, vertebrate cells, etc., can solve problems such as unpredictable negative effects, reduced beef quality, and unestablished beef cattle muscle cells.

Inactive Publication Date: 2019-04-09
INST OF ANIMAL SCI & VETERINARY MEDICINE SHANDONG ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

It is attributed to the reduction of beef quality caused by the stress caused by improper management and transportation of beef cattle during the process of feeding and slaughtering
At present, this problem has attracted widespread attention, but most studies are still only at the production level
In addition, many scholars and experts plan to add antioxidant active substances to beef cattle feed to improve beef quality, but due to the lack of molecular and cellular mechanism research, the dosage is blind, and the negative effects of feeding are difficult to predict
At present, the oxidative damage model of beef cattle muscle cells has not been established in China

Method used

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  • Construction method of oxidative damage model for muscle cell of beef cattle and application of oxidative damage model
  • Construction method of oxidative damage model for muscle cell of beef cattle and application of oxidative damage model
  • Construction method of oxidative damage model for muscle cell of beef cattle and application of oxidative damage model

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Embodiment Construction

[0048] In order to clearly illustrate the technical features of the solution, the solution will be described below through specific implementation modes.

[0049] see Figure 1 to Figure 3 , the present invention is: a method for constructing a beef cattle muscle cell oxidative damage model, comprising the following steps:

[0050] Step 1: Prepare cell culture medium:

[0051] 50-60 parts (parts by volume) of fetal bovine serum (FBS), 2-3 parts (parts by volume) of penicillin and streptomycin mixed solution are dissolved in 450-500 parts (parts by volume) of cell culture medium, and after 0.22 Divide into sterilized centrifuge tubes after filtering with a micron filter membrane, 40-45 ml per tube, store at 4°C, preheat the culture solution in a 37°C water bath 0.5-1 hour before cell culture, and obtain cell culture liquid;

[0052] Step 2: Separation of beef cattle muscle tissue:

[0053] Cut 10g-20g of ventral muscle tissue of adult beef cattle, rinse 2-3 times with steri...

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Abstract

The invention provides a construction method of an oxidative damage model for a muscle cell of a beef cattle and the application of the oxidative damage model, and belongs to the technical field of cytobiology. The invention adopts the technical scheme that the construction method of the oxidative damage model for the muscle cell of the beef cattle comprises the following steps: preparing a cell culture fluid, separating a beef cattle muscle tissue, culturing the muscle cell of the beef cattle, and acquiring the construction parameter of the oxidative damage model. The construction method hasthe beneficial effects that the separated muscle cell of the beef cattle is taken as the object of study, the oxidative damage model for the muscle cell of the beef cattle is established through hydrogen peroxide, the materials are provided for studying the reduction of the beef quality and studying the nutrition control mechanism, the research and development costs for the subsequent tests are reduced; the established oxidative damage model for the muscle cell of the beef cattle provides the mechanism studying materials for studying the control mechanism of the beef quality and developing anantioxidant active material, and the time and the cost for researches and development are greatly shortened and reduced by directly applying the model.

Description

technical field [0001] The invention relates to the field of cell biology and technology, in particular to a method for constructing a beef cattle muscle cell oxidative damage model and its application. Background technique [0002] With the improvement of people's living standards, beef has entered thousands of households, but the quality varies widely. It is attributed to the reduction of beef quality caused by the stress caused by improper management and transportation of beef cattle during the process of feeding and slaughtering. At present, this problem has attracted widespread attention, but most researches are still only at the production level. In addition, many scholars and experts plan to add antioxidant active substances to beef cattle feed to improve beef quality, but due to the lack of molecular and cellular mechanism research, the dosage of additives is blind, and the negative effects of feeding are difficult to predict. At present, the oxidative damage model...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/077C12Q1/02C12Q1/26C12Q1/30
CPCC12N5/0652C12Q1/26C12Q1/30G01N33/5014G01N33/5061
Inventor 张相伦游伟靳青谭秀文刘桂芬赵红波魏晨刘晓牧万发春刘倚帆
Owner INST OF ANIMAL SCI & VETERINARY MEDICINE SHANDONG ACADEMY OF AGRI SCI
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