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Method for detecting content of trans-anethole in fennel

A trans-anethole and detection method technology, which is applied in the field of detection of trans-anethole content in cumin, can solve the problems of low resolution and interference in sample detection, and achieve high sensitivity, high accuracy and high stability Effect

Inactive Publication Date: 2019-04-05
成都康美药业生产有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Different chromatographic instruments, different chromatographic columns, and different chromatographic parameters will cause interference in sample detection and low resolution

Method used

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  • Method for detecting content of trans-anethole in fennel
  • Method for detecting content of trans-anethole in fennel
  • Method for detecting content of trans-anethole in fennel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A method for detecting trans-anethole content in fennel, comprising:

[0039] Control solution preparation steps: take trans-anethole reference substance, add ethyl acetate to prepare a control solution, each 1mL control solution contains 0.4mg trans-anethole reference substance;

[0040] Preparation steps of the test solution: Take 0.4 g of fennel powder passed through the No. 3 sieve, add 20 mL of ethyl acetate, weigh it, obtain the weight A, and conduct ultrasonic treatment for 26 minutes. The parameters of ultrasonic treatment are: power 240W, frequency 40kHz, Allow to cool, weigh again to obtain weight B, make up the weight lost from A to B with ethyl acetate, shake well, filter, and take the filtrate to obtain the test solution;

[0041] Determination steps: Take 2 μL of the control solution and the test solution respectively, inject them into the gas chromatograph, and measure them.

[0042] Wherein, the chromatographic column of the gas chromatograph is a polye...

Embodiment 2

[0044] A method for detecting trans-anethole content in fennel, comprising:

[0045] Control solution preparation steps: take trans-anethole reference substance, add ethyl acetate to prepare a control solution, each 1mL control solution contains 0.4mg trans-anethole reference substance;

[0046] Preparation steps of the test solution: take 0.5 g of cumin powder passed through the No. 3 sieve, add 25 mL of ethyl acetate, weigh it, and obtain the weight A, and perform ultrasonic treatment for 29 minutes. The parameters of ultrasonic treatment are: power 270W, frequency 40kHz, Allow to cool, weigh again to obtain weight B, make up the weight lost from A to B with ethyl acetate, shake well, filter, and take the filtrate to obtain the test solution;

[0047] Determination steps: Take 2 μL of the control solution and the test solution respectively, inject them into the gas chromatograph, and measure them.

[0048] Wherein, the chromatographic column of the gas chromatograph is a po...

Embodiment 3

[0050] A method for detecting trans-anethole content in fennel, comprising:

[0051] Control solution preparation steps: take trans-anethole reference substance, add ethyl acetate to prepare a control solution, each 1mL control solution contains 0.4mg trans-anethole reference substance;

[0052] Preparation steps of the test solution: take 0.6g of fennel powder passed through the No. 3 sieve, add 30mL of ethyl acetate, weigh it, and obtain the weight A, and perform ultrasonic treatment for 34 minutes. The parameters of ultrasonic treatment are: power 300W, frequency 40kHz, Allow to cool, weigh again to obtain weight B, make up the weight lost from A to B with ethyl acetate, shake well, filter, and take the filtrate to obtain the test solution;

[0053] Determination steps: Take 2 μL of the control solution and the test solution respectively, inject them into the gas chromatograph, and measure them.

[0054] Wherein, the chromatographic column of the gas chromatograph is a pol...

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Abstract

The invention discloses a method for detecting the content of trans-anethole in fennel. The method includes the steps: a contrast solution is prepared, specifically, trans-anethole reference substanceis taken and added with ethyl acetate to prepare the contrast solution; a tested solution is prepared, specifically, a fennel sample is taken and added with the ethyl acetate, the mixture is weightedto obtain a weight A, after ultrasonic treatment and chilling, the mixture is weighted again to obtain a weight B, the ethyl acetate is used for making up the weight lost from the A to the B, a subsequent filtrate is taken after shaking up and filtering, and the tested solution is obtained; and determination is conducted, specifically, the contrast solution and the tested solution are taken correspondingly and injected into a gas chromatograph for determination, and the trans-anethole content can be detected. According to the detection method, the degree of separation is high, matrix interference can be resisted, sensitivity is high, stability is high, and the accuracy is high.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicine detection, in particular to a method for detecting the content of trans-anethole in cumin. Background technique [0002] At present, my country's supervision on the quality of Chinese herbal medicines and their decoction pieces has been continuously improved, and Chinese medicine has also been favored by the general public again. Chinese medicine is more and more widely used in China, and the quality of Chinese medicine is also receiving more and more attention. The effective content of characteristic components in medicinal materials and decoction pieces is one of the important indicators to evaluate and affect their quality. For fennel, the content of trans-anethole is an important indicator for detecting and evaluating its quality. At present, the detection method for the detection of trans-anethole content in cumin is gas chromatography. The pretreatment and extraction of samples m...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 许冬瑾钟世珍
Owner 成都康美药业生产有限公司
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