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Breaking method of desert algae cells

A crushing method and desert algae technology, applied in grain processing and other directions, can solve the problem of inability to crush desert algae, and achieve the effects of avoiding local high temperature phenomenon, preventing denaturation and inactivation, and efficiently crushing

Inactive Publication Date: 2019-04-05
新疆拓必达科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current conventional method of ultrasonically breaking algae cell walls cannot break the cell walls of desert algae and allow the active ingredients to come out.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for ultrasonic crushing of desert algae cells, specifically comprising the following steps:

[0023] (1) Measure 80ml of the cultured desert algae cell solution, put it in a 400ml beaker, add water to dilute it, and place the beaker in an ice bath. Adding water to dilute desert algae cells can improve the ultrasonic effect.

[0024] (2) Put the ultrasonic head into the middle region of the desert algae cell liquid, set the ultrasonic frequency to 60kHz, and set the power to 300W for ultrasonic treatment, and calculate the cell fragmentation rate by the number of complete cells observed under the microscope before and after fragmentation, and Each condition is counted 3 times on average.

[0025] (3) Turn on the power supply of the ultrasonic generator, set the ultrasonic time to 15 minutes, the interval time to 3 seconds, and the number of cycles to 60 times to obtain the broken desert algae cell liquid.

[0026] After collecting the broken desert algae cell ...

Embodiment 2

[0030] A method for ultrasonic crushing of desert algae cells, specifically comprising the following steps:

[0031] (1) Measure 80ml of the cultured desert algae cell solution, put it in a 400ml beaker, add water to dilute it, and place the beaker in an ice bath.

[0032] (2) Put the ultrasonic head into the middle area of ​​the desert algae cell liquid, and set the ultrasonic frequency to 60kHz and the power to 500W for ultrasonic treatment, and calculate the cell fragmentation rate by the number of complete cells observed under the microscope before and after fragmentation, and Each condition is counted 3 times on average.

[0033] (3) Turn on the power of the ultrasonic generator, set the ultrasonic time to 20 minutes, the interval time to 3 seconds, and the number of cycles to 70 times to obtain the broken desert algae cell liquid.

[0034] After collecting the broken desert algae cell liquid, measure the solution temperature, soluble COD, and chlorophyll content. The r...

Embodiment 3

[0037] A method for ultrasonic crushing of desert algae cells, specifically comprising the following steps:

[0038] (1) Measure 80ml of the cultured desert algae cell solution, put it in a 400ml beaker, add water to dilute it, and place the beaker in an ice bath.

[0039] (2) Put the ultrasonic head into the middle region of the desert algae cell liquid, and set the ultrasonic frequency to 60kHz, and the power to 700W for ultrasonic treatment, and calculate the cell fragmentation rate by the number of complete cells observed under the microscope before and after crushing, each Conditions were counted 3 times on average.

[0040] (3) Turn on the power supply of the ultrasonic generator, set the ultrasonic time to 25 minutes, the interval time to 3 seconds, and the number of cycles to 80 times to obtain the broken desert algae cell liquid.

[0041] After collecting the broken desert algae cell liquid, measure the solution temperature, soluble COD, and chlorophyll content. The...

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PUM

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Abstract

The invention discloses a breaking method of desert algae cells. The breaking method of the desert algae cells comprises the steps that the desert algae cell sap is subjected to ultrasonic breaking treatment, the ultrasonic frequency is 60 kHz, the power ranges from 300 to 800 W, time of each treatment ranges from 15 to 35 min, and treatment is carried out for 60 to 90 times. According to the breaking method of the desert algae cells, the ultrasonic breaking method is adopted, and the method is related with ultrasonic time, cell sap concentrations, and sound frequency and sound energy of ultrasonic waves. Ultrasonic wave extracting process parameters are researched, the process technology with the high extraction rate is optimized, the desert algae cells can be effectively and efficientlybroken, ultrasonic treatment is carried out under the ice-bath condition, the local high temperature phenomenon generated by the ultrasonic treatment can be avoided, meanwhile, the ultrasonic mode selects the intermittent manner, and denaturation and inactivation of heat-reactivity active matter can be prevented.

Description

technical field [0001] The invention specifically relates to a method for crushing desert algae cells. Background technique [0002] Desert algae refers to a type of algae isolated from desert soil and artificially cultivated purely. Because its habitat is a harsh desert environment, people are accustomed to collectively refer to this type of algae as desert algae. [0003] Biological and nutritional studies on desert algae cells have found that such cells not only have unique nutritional characteristics, but also have a very ancient developmental status in biological evolution, which fully demonstrates that the Taklamakan Desert was once a vast ocean. With the disappearance of the ocean, the seaweed gradually evolved into today's desert algae. [0004] Desert algae is a specific ecological group of terrestrial algae, mainly referring to algal organisms that grow in desert areas. As pioneer colonizing organisms, desert algae can grow and reproduce in harsh environments (su...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B02C19/18
CPCB02C19/18
Inventor 艾尼瓦尔·艾白都拉亚力坤·牙生
Owner 新疆拓必达科技发展有限公司
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