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Method for inducing adventitious buds for rapid propagation of zingiberaceae seedlings

A plant and seedling technology, which is applied in the field of rapid propagation of Zingiberaceae seedlings by inducing adventitious buds, can solve the problems of high mutation rate, uneven ginger block, high cost, etc., and achieve the effect of high multiplication efficiency and short cycle

Active Publication Date: 2019-04-05
SOUTHWEAT UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, in production, germination is usually used to induce the germination of ginger pieces, and after cutting the stem tip, a sterile system is established through surface disinfection. The disadvantages of this method are: ① time-consuming, germination often needs to wait for 1-2 weeks, from germination to outside It usually takes 6-8 months to establish the implant; ②The cost is high, and it is often necessary to provide an environment above 20°C for germination in winter, and the production needs to increase the room temperature by burning boilers and electricity; ③The pollution rate is high, and the ginger block is uneven Uneven, surface disinfection is not easy to complete, resulting in a high pollution rate
However, there are problems with this method: ① With the increase of the number of subcultures, the regeneration ability of the callus is significantly degraded; ② After the callus stage, the mutation rate is high
Therefore it can be seen that a safe, easy and fast propagation method is extremely lacking in the production of Zingiberaceae plants at present.

Method used

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  • Method for inducing adventitious buds for rapid propagation of zingiberaceae seedlings
  • Method for inducing adventitious buds for rapid propagation of zingiberaceae seedlings
  • Method for inducing adventitious buds for rapid propagation of zingiberaceae seedlings

Examples

Experimental program
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Effect test

Embodiment 1

[0033]Select 5-week-old robust ginger variety 'Sichuan Qianwei Huangkoujiang' tissue culture seedlings, keep 1.0-2.0cm tap root under ultra-clean workbench conditions, remove the rest of the peripheral roots; keep the tap root and 1-1.5 cm above the enlarged stem, Remove the remaining stems and leaves, and keep the base leaf sheath 1.0-2.0cm after the leaves are cut off; cut the enlarged stem transversely, the thickness of each thin layer is 2.0-3.0mm, cut each leaf sheath into 5.0-7.0mm, and inoculate in On the induction medium of MS+TDZ 2.0mg / L+NAA 1.0mg / L, place it in a light culture room at 25±2°C for 15 days; transfer the obtained adventitious buds to 6-BA 2.0mg / L +NAA0.5mg / L MS medium, placed in a light culture room at 25±2°C for 4 weeks, the proliferation rate of adventitious buds was 6.

Embodiment 2

[0035] Select the robust ginger variety 'Leshan Xiba Jiang' tissue culture seedlings with a seedling age of 5 weeks, keep the main root of 1.0-2.0 cm under the condition of ultra-clean workbench, and remove the remaining peripheral roots; keep the main root and the enlarged stem of 1-1.5 cm above, remove For the remaining stems and leaves, keep the base leaf sheath at 1.0--2.0cm after the leaves are cut off; cut the enlarged stem transversely, the thickness of each thin layer is 2.0-3.0mm, cut each leaf sheath into 5.0-7.0mm, and inoculate in On the induction medium of MS+TDZ 2.0mg / L+NAA 1.0mg / L, place it in a light culture room at 25±2°C for 15 days; transfer the obtained adventitious buds to 6-BA 2.0-3.0mg / L+NAA0.2-0.5mg / L MS medium, placed in a light culture room at 25±2°C for 4 weeks, the proliferation rate of adventitious buds is 4-6.

Embodiment 3

[0037] Select 5-week-old robust ginger variety 'Sichuan Bamboo Root Ginger' tissue-cultured seedlings, keep the 1.0-2.0cm tap root under ultra-clean workbench conditions, and remove the rest of the peripheral roots; keep the tap root and 1-1.5 cm above the swollen stem, remove For the remaining stems and leaves, keep the base leaf sheath at 1.0--2.0cm after the leaves are cut off; cut the enlarged stem transversely, the thickness of each thin layer is 2.0-3.0mm, cut each leaf sheath into 5.0-7.0mm, and inoculate in On the induction medium of MS+TDZ 3.0mg / L+NAA 0.5mg / L, place it in a light culture room at 25±2°C for 15 days; transfer the obtained adventitious buds to 6-BA 2.0mg / L +NAA0.5mg / L MS medium, placed in a light culture room at 25±2°C for 4 weeks, the proliferation rate of adventitious buds was 4.

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Abstract

The invention discloses a method for inducing adventitious buds for rapid propagation of zingiberaceae seedlings. The method comprises the following steps of selecting robust ginger tissue culture seedlings, and removing peripheral roots; removing main stems, keeping roots and expanding stems, and keeping basic sheath parts of leaves; transversely cutting the expanding stems and the sheath parts respectively, inoculating the cut expanding stems and sheath parts onto an inducing culture medium, and conducting illumination culture; transferring the adventitious buds to a proliferation culture medium, and conducting illumination culture. The novel method for the rapid propagation of the zingiberaceae seedlings is established, the seedlings are directly formed, a callus stage is not involved,there is no risk of variation, the cycle is short, and the efficiency is high.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture rapid propagation, in particular relates to a method for inducing adventitious buds to carry out rapid propagation of Zingiberaceae seedlings. Background technique [0002] Zingiberaceae plants are widely distributed in tropical and subtropical regions of the world. There are 22 genera and 209 species of Zingiberaceae plants in my country, mainly distributed in the southern and southwestern provinces, among which Guangdong, Guangxi and Yunnan are the most abundant. Zingiberaceae plants are a treasure house of plant resources, and our country has a long history of understanding and utilizing Zingiberaceae plants. Ginger plants have many functions and are indispensable in life. For example, ginger is called one of the "four spicy" in the food kingdom. It contains a variety of ingredients and is rich in nutrition. It is not only an important seasoning vegetable, but also used as a seaso...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 王仁睿唐志康周新力黄晶曾明颖侯大斌刘成才王丽君袁铭悦帕坦姆汗·阿布杜合力克
Owner SOUTHWEAT UNIV OF SCI & TECH
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