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Digital microfluidic chip and pathogen immunoassay method based thereon

An immunoassay method and microfluidic chip technology, which are applied in chemical instruments and methods, measuring devices, and laboratory containers, etc., can solve the problems of limited detection effect, time-consuming, cumbersome operation, etc., and achieve combination speed and combination Improved efficiency, improved detection sensitivity, and simple peripheral equipment

Inactive Publication Date: 2019-04-02
DALIAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The current automated immunoassay instruments have disadvantages such as cumbersome operation, slow response, long time consumption, large consumption of reagents and samples, and high detection cost.
At the same time, the detection equipment has a complex structure, relies on high-precision manufacturing technology, is expensive, and bulky, and requires trained professionals to operate
In addition, the current automated immunoassay instruments are mainly designed for batch testing, and most instruments only provide 2-3 emergency testing channels, which is poor in flexibility and not suitable for the detection of sporadic samples
With the introduction of concepts such as point-of-care, home health tests, and tele-diagnosis, as well as the development of community hospitals, the existing automated immune testing equipment can no longer meet the requirements The demand of modern medical development model
The test strip detection represented by colloidal gold technology is convenient to use, but its detection target is limited and the detection sensitivity is low, and the detection effect is limited.

Method used

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  • Digital microfluidic chip and pathogen immunoassay method based thereon
  • Digital microfluidic chip and pathogen immunoassay method based thereon

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Embodiment

[0035] This embodiment provides a method for detecting pathogen immunity. The detection method uses a digital microfluidic chip. The microfluidic chip is provided with a plurality of electrodes, specifically, the lower microfluidic chip includes a base layer 1, and the plurality of electrodes are laid on the surface of the base layer at intervals, and an insulating layer 2 is formed on the electrodes. , a gold film 6 is deposited on the surface of the insulating layer 2 on the detection electrode. In this embodiment, preferably, the base layer 1 is a glass base layer, and the insulating layer is SiO 2 Insulation layer, the conductive glass is ITO conductive glass.

[0036] The plurality of electrodes include a reagent electrode corresponding to form a reagent storage tank 10, a waste liquid electrode forming a waste liquid storage tank 9, a PBS electrode forming a PBS pool 8, a detection electrode forming a detection area 12, and an input electrode forming a droplet running c...

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Abstract

The invention relates to the technical field of microfluidic chips, in particular to a digital microfluidic chip and a pathogen immunoassay method based thereon. The method comprises the following steps of: (1) making a digital microfluidic chip; (2) modifying the antigen in a detection region of the digital microfluidic chip; and (3) adding the sample and running the digital microfluidic chip, photographing and detecting a fluorescent signal through a fluorescence detection system CCD and performing a quantitative analysis. The digital microfluidic chip comprises a sample hole, a reagent electrode forming a reagent reservoir, a transport electrode forming a droplet operation channel, and a detection electrode forming a detection region. The digital microfluidic chip and the pathogen immunoassay method based thereon have short reaction time, high detection sensitivity, simple peripheral equipment, high flexibility and low energy consumption, can meet the needs of community hospitals, remote clinics and the like, are suitable for building portable analytics platforms, can be analyzed in batches or in a single sample, and has strong adaptability to the sample size.

Description

technical field [0001] The invention relates to the technical field of microfluidic chips, in particular to a digital microfluidic chip and a pathogen immune detection method based on it. Background technique [0002] Immunoassay is one of the most important detection methods in clinical testing at present. It is widely used in disease diagnosis, pathogen screening, drug detection, etc., and is also widely used in environmental monitoring, food safety and other fields. Since the 1980s, the introduction and improvement of automated detection technology has made automatic and high-throughput immunoassay possible, greatly promoting the application and promotion of immunoassay technology. [0003] The current automated immunoassay instruments have disadvantages such as cumbersome operation, slow response, long time consumption, large consumption of reagents and samples, and high detection cost. At the same time, the detection equipment has a complex structure, relies on high-pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/327G01N21/64B01L3/00
CPCB01L3/5027B01L2200/10G01N21/6486G01N27/26G01N27/3275
Inventor 王云华郑国侠卢玲
Owner DALIAN UNIV
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