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Guided tissue regeneration membrane for compositing BMSCs, patch and preparation method thereof

A technology to guide tissue regeneration and patches, applied in the direction of tissue regeneration, medical preparations containing active ingredients, drug combinations, etc., can solve the problem of promoting scar endometrium growth, increasing endometrial thickness, immune inflammation-induced infection, etc. problems, achieve the effect of increasing intimal thickness and local vascular density, good biocompatibility, and promoting smooth muscle repair

Inactive Publication Date: 2019-04-02
陕西佰瑞衡健康科技有限公司
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  • Application Information

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Problems solved by technology

Disadvantages: severe damage to the endometrium, unable to solve the problem of endometrial scars, unable to achieve functional repair of the endometrium, and very prone to re-adhesion; in addition, after the separation of intrauterine adhesions, fresh amniotic membrane is used to cover the wound. Disadvantages: the scar cannot be induced The membrane itself is functionally repaired, and the amniotic membrane also has risks such as immune inflammation and induced infection; in addition, uterine cavity perfusion of granulocyte colony-stimulating factor has been reported to increase the thickness of the endometrium in some patients with thin endometrium. Disadvantages: granulocytes cannot be retained Colony-stimulating factor is localized, with short acting time and poor local curative effect, and cannot achieve the effect of promoting the growth of scar intima; Evenly injected into the uterine cavity, given postoperative hormone replacement therapy, can increase the thickness of the endometrium of the patient. Disadvantages: After local injection of BMSCs, it is difficult to localize the local injury, which affects the curative effect

Method used

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  • Guided tissue regeneration membrane for compositing BMSCs, patch and preparation method thereof
  • Guided tissue regeneration membrane for compositing BMSCs, patch and preparation method thereof
  • Guided tissue regeneration membrane for compositing BMSCs, patch and preparation method thereof

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Embodiment Construction

[0041] The present invention will be described in further detail below in conjunction with specific embodiments and accompanying drawings, so that those skilled in the art can implement it with reference to the description.

[0042] It should be understood that terms such as "having", "comprising" and "including" used herein do not exclude the existence or addition of one or more other combinations.

[0043] A preparation method of a guided tissue regeneration film of composite BMSCs, comprising the following steps:

[0044] 1) Trypsinize the BMSCs in the growth phase to prepare a single-cell suspension, the number of cells in the single-cell suspension is 2.5×10 6 piece / cm 2 ;

[0045] 2) Take the single-cell suspension and evenly drop it onto the rough surface of the tissue regeneration membrane, and set aside;

[0046] 3) Put the guided tissue regeneration membrane with dropwise single cell suspension at a temperature of 37°C and contain 5% CO 2 In an incubator, culture...

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Abstract

The invention belongs to the technical field of obstetrical and gynecology, and more specifically relates to a guided tissue regeneration membrane for compositing BMSCs, a patch and a preparation method thereof. The method prepares a single cell suspension by trypsinizing the BMSCs in a growth phase, and uniformly drops the single cell suspension into the guided tissue regeneration membrane, and then cultures the material in an incubator to obtain the guided tissue regeneration membrane for compositing the mesenchymal stem cells. The BMSCs of the invention are used as an active component for treating endometrial damage, the obtaining is convenient, local microenvironment and immune regulation can be improved by secretion of growth factors, the guided tissue regeneration membrane has good biocompatibility, promotes damage to endometrial repair, and increases endometrium thickness and vascular density.

Description

technical field [0001] The invention belongs to the technical field of obstetrics and gynecology, and in particular relates to a composite BMSCs guided tissue regeneration film, a patch and a preparation method. Background technique [0002] When the endometrium is severely damaged, the functional repair of the endometrium is obstructed, replaced by connective tissue hyperplasia and repair, fibrosis of the anterior and posterior walls of the uterus, and scar formation. Clinically, the main manifestations are intrauterine adhesions, amenorrhea, and uterine infertility. There is no effective treatment. [0003] Before the present invention, the treatment method for severe endometrial injury is as follows: clinically general method is hysteroscopic operation mechanical separation of intrauterine adhesion, postoperative placement of intrauterine device or anti-adhesion material (mainly hyaluronic acid) Etc., and give estrogen after operation to promote intimal growth. Disadvan...

Claims

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Application Information

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IPC IPC(8): A61L31/16A61L31/00A61L27/38A61L27/54A61P15/00
CPCA61K35/28A61L27/3834A61L27/3882A61L27/54A61L31/005A61L31/16A61L2300/412A61L2430/22
Inventor 董玲娟明磊国李晓宁
Owner 陕西佰瑞衡健康科技有限公司
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