Separating medium for young and old red blood cells and preparation method and application thereof

Abstract

The invention discloses a separating medium for young and old red blood cells and a preparation method and application thereof. The separating medium is prepared from dimethyl phthalate and dibutyl phthalate according to a volume ratio of (0.95 to 1.05):(1.95 to 2.05). The separating medium is characterized in that the density of the reticulocyte or young red blood cell is 1.085, and the density of the old red blood cell is 1.114; by utilizing the characteristic that the dimethyl phthalate and dibutyl phthalate are not compatible with the red blood cell and do not injure the red blood cell, asolution with density between the densities of young and old red blood cells is prepared; in the high-speed centrifugal separation process, four layers are obviously formed in capillary tubes, the young and old red blood cells are completely separated, the accuracy in testing results is guaranteed, and the precision of the testing results is improved. The separating medium has the advantages thatthe used material is simple; when the separating medium is used for separating the young and old red blood cells, the required time is short, and the usage amount of blood is small; the result is visual, the error is small, and the separating medium can be widely applied to the conventional separation detection.

Description

technical field [0001] The invention belongs to the technical field of cell separation, and in particular relates to a separation medium for old and new red blood cells, a preparation method and application thereof. Background technique [0002] In the detection of accurate identification of blood type and investigation of clinical adverse reactions of blood transfusion, it is necessary to separate old and new red blood cells, and obtain a certain number of old and new red blood cells through the difference in the proportion of old and new red blood cells to identify blood types. At present, the separation of old and new red blood cells mainly adopts the method of capillary ultracentrifugation. The capillary ultracentrifugation method is mainly based on the difference in the density of old and new red blood cells due to the difference in the nuclear-to-cytoplasmic ratio of fresh cells and old cells. Layer separation, so as to achieve the purpose of separating new and old re...

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Problems solved by technology

[0003] The purpose of the present invention is to provide a separation medium for old and new red blood cells and its preparation method and application, so as to solve the problem that the boundary between old and new red blood cells is not obvious and the separation and detection results are inaccurate during the ultracentrifugation separation process

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