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Dual-response ratiometric fluorescent probe based on dual detection mechanism and its construction method

A ratiometric fluorescent probe and construction method technology, applied in the field of dual-response ratiometric fluorescent probes and their construction, which can solve the problem of less ratiometric probe construction.

Active Publication Date: 2022-07-22
LINYI UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, they are all fluorescent probes with a single fluorophore, and there are fewer ratio probes to be constructed, and fewer ratio probes to be constructed with dual response types

Method used

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  • Dual-response ratiometric fluorescent probe based on dual detection mechanism and its construction method
  • Dual-response ratiometric fluorescent probe based on dual detection mechanism and its construction method
  • Dual-response ratiometric fluorescent probe based on dual detection mechanism and its construction method

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Embodiment 1

[0045] The schematic diagram of the construction of dual-response ratiometric fluorescent probes based on the dual detection mechanism is shown in figure 1 , the specific construction steps are as follows:

[0046] 1. The carbon dots emitting blue light with a wavelength of 400 nm were prepared by microwave method.

[0047] Dissolve 0.3 g of leucine and 1 g of urea in 10 mL of water, microwave in a household microwave oven for 4 min to obtain a pale yellow solid, re-add 10 mL of water to dissolve it, and centrifuge (12,000 rpm, 10 min) to obtain a pale yellow supernatant, which is the prepared carbon dots. The carbon dots emit blue fluorescence under ultraviolet light, and the maximum emission wavelength is 400nm.

[0048] There are many preparation methods of carbon dots, many raw materials, and many emission wavelengths, which can be adjusted from 400-700 nm. In the present invention, the emission wavelength of the carbon dots is required to be the same as the absorption ...

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Abstract

The invention provides a dual-response ratio fluorescent probe constructed based on a dual detection mechanism. First, a ratio fluorescent probe based on silver etching is constructed, and then H is added to the ratio fluorescent probe based on silver etching. 2 O 2 , a dual-response ratiometric fluorescent probe based on a dual-detection mechanism was obtained. The invention effectively combines the two response modes of the inner filter effect and the electron transfer for the first time, and adopts the dual response mode to construct a ratio fluorescent probe with high detection sensitivity, which can quantitatively detect H 2 O 2 concentration and glucose concentration.

Description

technical field [0001] The present invention relates to a dual-response ratio fluorescent probe constructed based on a dual detection mechanism and a construction method thereof. Background technique [0002] Compared with single-response fluorescent probes, ratiometric fluorescent probes have the advantages of high sensitivity, low background, and visual detection. Ratiometric fluorescent probes can be divided into reference type according to the response mode of the fluorophore (two fluorophores, only the fluorescence intensity of one fluorophore changes with the addition of the detected substance, and the fluorescence intensity of the other fluorophore always remains unchanged. , referred to as reference) and dual-response type (the fluorescence intensity of both fluorophores changes with the addition of the analyte. And the direction of change is opposite, one increases while the other decreases). Compared with the reference type, the ratiometric fluorescent probe of th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/06C09K11/88G01N21/64
CPCG01N21/6428G01N21/643C09K11/06C09K11/88
Inventor 徐守芳陆宏志
Owner LINYI UNIVERSITY
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