Phage water aqua preservative, preparation method and application thereof

A technology of phage and antiseptic, applied in the field of phage water preservative and its preparation, which can solve the problems of troublesome preparation process, limited types of phage and its preparations, and high cost, and achieve stable phage titer, low cost, and improved stability Effect

Inactive Publication Date: 2019-03-26
PHAGELUX (NANJING) BIO-TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the above-mentioned patents use many components, the cost is high, the preparation process is cumbersome, and the types of applicable bacteriophages and their preparations are limited.

Method used

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  • Phage water aqua preservative, preparation method and application thereof
  • Phage water aqua preservative, preparation method and application thereof
  • Phage water aqua preservative, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0037] Application experiment of preservatives to Vibrio parahaemolyticus phage VP46 under standing state: take two groups with a titer of 5.0x10 10500ml of Vibrio parahaemolyticus phage VP46 with PFU / ml was placed in an environment of normal temperature 37°C in an open form. Add preservatives with a final concentration of 1.5g / L sodium dehydroacetate and 5g / L chitosan to one of the groups. The two groups of Vibrio parahaemolyticus phage VP46 preparations were allowed to stand still, samples were taken and spread every 8 hours, and the bacterial content was determined until 72 hours. At the same time, samples were taken every 8 hours to determine the titer of Vibrio parahaemolyticus phage VP46.

[0038] Potency determination method:

[0039] Use SM solution as the diluent, and dilute Vibrio parahaemolyticus phage VP46 step by step to l0 7 times. Take l0 respectively 5 , l0 6 and l0 7 1000 μl of the diluted phage culture solution was evenly mixed with 300 μl of the host ...

Embodiment 2

[0045] Application experiment of preservatives to Vibrio parahaemolyticus phage VP46 under shaking culture state: Take two groups with a titer of 5.0x10 10 500ml of PFU / ml Vibrio parahaemolyticus phage VP46 was exposed to 37°C at room temperature, and preservatives with a final concentration of 1.5g / L sodium dehydroacetate and 5g / L chitosan were added to one of the groups. Two groups of Vibrio parahaemolyticus phage VP46 were shaken at 150 rpm, samples were taken every 8 hours, and the bacterial content was measured until 72 hours. At the same time, samples were taken every 8 hours to determine the titer of Vibrio parahaemolyticus phage VP46. Potency determination method:

[0046] Use SM solution as the diluent, and dilute Vibrio parahaemolyticus phage VP46 step by step to l0 7 times. Take l0 respectively 5 , l0 6 and l0 7 1000 μl of the diluted phage culture solution was evenly mixed with 300 μl of the host bacteria Vibrio parahaemolyticus HN9 bacteria solution, and all...

experiment example 3

[0052] Application experiment of preservatives to Vibrio parahaemolyticus phage VP48 under standing state: take two groups with a titer of 5.0x10 10 500ml of Vibrio parahaemolyticus phage VP48 with PFU / ml was placed in an environment of normal temperature 37°C in an open form. Add preservatives with a final concentration of 1.5g / L sodium dehydroacetate and 5g / L potassium sorbate to one of the groups. Let the two groups of Vibrio parahaemolyticus phage VP48 stand still, take samples and spread them every 8 hours, and measure the bacterial content until 72 hours. At the same time, samples were taken every 8 hours to determine the titer of Vibrio parahaemolyticus phage VP48.

[0053] Potency determination method:

[0054] Use SM solution as the diluent, and dilute Vibrio parahaemolyticus phage VP48 step by step to l0 7 times. Take l0 respectively 5 , l0 6 and l0 7 1000 μl of the diluted phage culture solution was evenly mixed with 300 μl of the host bacteria Vibrio parahae...

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Abstract

The invention discloses a phage water aqua preservative, a preparation method and an application thereof and belongs to the technical field of microbial antisepsis. The technical scheme is characterized in that the phage water aqua preservative comprises the following components by weight: 1-3 parts of sodium dehydroacetate, 7-9 parts of preservative and the balance of water aqua. The phage wateraqua preservative is applicable to strains such as a vibrio parahaemolyticus phage, an escherichia coli phage, a salmonella phage, a ralstonia solanacearum phage, a staphylococcus aureus phage, an aeromonas hydrophila phage, the ralstonia solanacearum phage, an axonopus scoparius xanthomonas and an agrobacterium tumefaciens. The preservative disclosed by the invention has the advantages of preventing bacterial growth, being non-toxic to the phages and improving the stability of the phages so that the phages are difficult to deteriorate and the stability of the titer is maintained. In a practical case, the preservative can play a good role in the prevention and control of animal and plant diseases, and is especially applicable to the industrial large-scale production of phage preparations and the process of user storage and field application.

Description

technical field [0001] The invention relates to the technical field of microbial antiseptic, more specifically, it relates to a bacteriophage aqueous preservative and its preparation method and application. Background technique [0002] Phage is a type of virus capable of infecting bacteria. It is small in size and simple in structure. It is mainly composed of protein capsid and nucleic acid wrapped inside. Bacteriophages are strictly parasitic, widely distributed in nature, can specifically lyse bacteria, have good safety, and have no toxic and side effects on the body and the environment. [0003] Phage is an important experimental tool and the most ideal material in the field of molecular biology. It can be used to prevent and treat diseases, identify bacterial types, screen and detect carcinogens, etc. But phages are very fragile and can go bad very easily. How to do a good job in the preservation of bacteriophage and its preparations so as to ensure its long-term pres...

Claims

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Application Information

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IPC IPC(8): A01N63/02A01P1/00A61K35/76A61P31/04
CPCA01N63/10A61K35/76A61P31/04Y02A50/30
Inventor 乔欢徐旭凌陈海费文斌黄杰胡怿林何四龙丛郁肖逍丁良许文建樊小九王卫斌沈婵娟
Owner PHAGELUX (NANJING) BIO-TECH CO LTD
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