Phage water aqua preservative, preparation method and application thereof
A technology of phage and antiseptic, applied in the field of phage water preservative and its preparation, which can solve the problems of troublesome preparation process, limited types of phage and its preparations, and high cost, and achieve stable phage titer, low cost, and improved stability Effect
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experiment example 1
[0037] Application experiment of preservatives to Vibrio parahaemolyticus phage VP46 under standing state: take two groups with a titer of 5.0x10 10500ml of Vibrio parahaemolyticus phage VP46 with PFU / ml was placed in an environment of normal temperature 37°C in an open form. Add preservatives with a final concentration of 1.5g / L sodium dehydroacetate and 5g / L chitosan to one of the groups. The two groups of Vibrio parahaemolyticus phage VP46 preparations were allowed to stand still, samples were taken and spread every 8 hours, and the bacterial content was determined until 72 hours. At the same time, samples were taken every 8 hours to determine the titer of Vibrio parahaemolyticus phage VP46.
[0038] Potency determination method:
[0039] Use SM solution as the diluent, and dilute Vibrio parahaemolyticus phage VP46 step by step to l0 7 times. Take l0 respectively 5 , l0 6 and l0 7 1000 μl of the diluted phage culture solution was evenly mixed with 300 μl of the host ...
Embodiment 2
[0045] Application experiment of preservatives to Vibrio parahaemolyticus phage VP46 under shaking culture state: Take two groups with a titer of 5.0x10 10 500ml of PFU / ml Vibrio parahaemolyticus phage VP46 was exposed to 37°C at room temperature, and preservatives with a final concentration of 1.5g / L sodium dehydroacetate and 5g / L chitosan were added to one of the groups. Two groups of Vibrio parahaemolyticus phage VP46 were shaken at 150 rpm, samples were taken every 8 hours, and the bacterial content was measured until 72 hours. At the same time, samples were taken every 8 hours to determine the titer of Vibrio parahaemolyticus phage VP46. Potency determination method:
[0046] Use SM solution as the diluent, and dilute Vibrio parahaemolyticus phage VP46 step by step to l0 7 times. Take l0 respectively 5 , l0 6 and l0 7 1000 μl of the diluted phage culture solution was evenly mixed with 300 μl of the host bacteria Vibrio parahaemolyticus HN9 bacteria solution, and all...
experiment example 3
[0052] Application experiment of preservatives to Vibrio parahaemolyticus phage VP48 under standing state: take two groups with a titer of 5.0x10 10 500ml of Vibrio parahaemolyticus phage VP48 with PFU / ml was placed in an environment of normal temperature 37°C in an open form. Add preservatives with a final concentration of 1.5g / L sodium dehydroacetate and 5g / L potassium sorbate to one of the groups. Let the two groups of Vibrio parahaemolyticus phage VP48 stand still, take samples and spread them every 8 hours, and measure the bacterial content until 72 hours. At the same time, samples were taken every 8 hours to determine the titer of Vibrio parahaemolyticus phage VP48.
[0053] Potency determination method:
[0054] Use SM solution as the diluent, and dilute Vibrio parahaemolyticus phage VP48 step by step to l0 7 times. Take l0 respectively 5 , l0 6 and l0 7 1000 μl of the diluted phage culture solution was evenly mixed with 300 μl of the host bacteria Vibrio parahae...
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