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Insect antifeedant 15β-hydroxyklaineanone transported in plants and its application

An insect and plant technology, applied in the fields of plant growth regulators, applications, and repellents, can solve problems such as loss of efficacy and interference effects, and achieve the effects of environmental friendliness, high antifeedant activity, and convenient use.

Active Publication Date: 2020-12-29
POMOLOGY RES INST GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, some compounds have been found to have antifeedant activity against insects, which are generally applied on the surface of plants to exert antifeedant activity, but this type of antifeedant needs to be sprayed, and it is easily affected by the interference of the external environment after application, such as rainwater washing will lead to the loss of drug efficacy, etc., so the development of an insect-feeding compound that can be transported in plants is one of the solutions to the problem

Method used

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  • Insect antifeedant 15β-hydroxyklaineanone transported in plants and its application
  • Insect antifeedant 15β-hydroxyklaineanone transported in plants and its application
  • Insect antifeedant 15β-hydroxyklaineanone transported in plants and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 115

[0036]Example 115 The plant systemic properties of β-hydroxyklaineanone and its antifeedant activity after systemic absorption

[0037]Method: Dissolve 15β-hydroxyklaineanone with methanol, then prepare the solution to a final concentration of 100μg / mL, and spread it evenly on the surface of a 1.2 cm diameter circular cabbage leaf disc. The amount of medicine applied to each leaf disc When the methanol on the surface of the leaf disc is naturally dried, the 3rd instar larvae of Plutella xylostella (Linnaeus) are inserted. The treatments at each concentration are repeated 3 times at a relative humidity of 50% to 70% and a temperature of 25°C to 28 After keeping in an environment of ℃ for 24 hours, the feeding area was counted, and the feeding rate and refusal rate were calculated.

[0038]The antifeedant activity of 15β-hydroxyklaineanone on the first instar larvae of Spodopteralitura (Fabricius) was tested with cassava leaves; the antifeedant activity of 15β-hydroxyklaineanone on the firs...

Embodiment 2

[0046]Example 2 15β-hydroxyklaineanone can be absorbed into the roots of Shanghai greens from the growing environment of Shanghai greens through the roots, and is transported upwards to the leaves, so that insects refuse to eat greens green leaves

[0047]Method: Dissolve 15β-hydroxyklaineanone in DMSO to prepare a mother liquor of 10000μg / mL, then use 0.5mM CaCl2The aqueous solution dilutes the mother liquor to a 100μg / mL culture solution. Place the pre-cultivated Shanghai green seedlings (6 leaf stage, not treated with 15β-hydroxyklaineanone) in 0.5mM CaCl2After pre-incubating in an aqueous solution for 2 hours, transfer it to the above-mentioned prepared 15β-hydroxyklaineanone culture solution. After 24 hours, take 0.5 g of the apical leaves of the seaweed, mash it and dissolve it in 2 mL of methanol. The liquid chromatography-mass spectrometer can be used to It was detected that the leaf extract contained 15β-hydroxyklaineanone. According to calculations, the content of 15β-hydroxy...

Embodiment 3

[0049]Example 3: 15β-hydroxyklaineanone can be absorbed from the growth environment of the Chinese cabbage through the roots into the inside of the Chinese cabbage root, and transported upwards to the leaves, so that the vegetable aphid Lipaphiserysimi can resist eating the cabbage leaves

[0050]Method: Dissolve 15β-hydroxyklaineanone in methanol to prepare a mother liquor of 10000μg / mL, then use 0.5mM CaCl2The aqueous solution dilutes the mother liquor to a 100μg / mL culture solution. Place the previously cultivated cabbage seedlings (6 leaf stage, not treated with 15β-hydroxyklaineanone) in 0.5mM CaCl2Pre-incubate in aqueous solution for 2 hours and then transfer to the above prepared 15β-hydroxyklaineanone culture medium. After 24 hours, insert wingless cabbage aphids, 100 per cage, repeat 4 times, and count the control aphid 24 hours later. The calculation formula for the number of aphids living on the leaves and the treated leaves, and the antifeedant rate is as follows:

[0051]

[005...

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Abstract

The invention discloses an insect antifeedant 15beta-hydroxyklaineanone conducted in plants and application. The 15beta-hydroxyklaineanone has antifeeding activity on various insects and can be conducted in the plants; the antifeeding effect is better than that of the antifeedant applied to the surfaces of the plants; the 15beta-hydroxyklaineanone can be used for preparing a systemic insect antifeedant, is more convenient to use, is not influenced by interferences of an external environment and has a good application prospect; the insect antifeedant is separated and purified from natural plants and has low toxicity, no residues, no pollution and no nuisance on economic crops including various grains, melons and vegetables; a series of problems produced by the drug resistance caused by traditional insecticide in the prior art can be solved.

Description

Technical field[0001]The invention belongs to the field of biological pesticides and plant protection, and specifically relates to an insect antifeedant 15β-hydroxyklaineanone that is transported in plants and its application.Background technique[0002]Agricultural pests occur on a large scale in many areas of our country, seriously jeopardizing agricultural safety, and even causing land, water, and air pollution problems. At present, chemical pesticide control is still the main measure, and the dosage and frequency of control are continuously added, which makes insect resistance develop rapidly. The development of resistance to pesticides makes pest control work difficult. Therefore, it is particularly important to explore efficient, safe and environmentally friendly green pesticides. The development of plant-derived pesticides has always been a research focus in the field of pesticides.[0003]In the long-term co-evolution of plants and insects, they use secondary compounds to resist...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/16A01P17/00
CPCA01N43/16
Inventor 邵雪花匡石滋赖多肖维强赵天艺凡超王晓容
Owner POMOLOGY RES INST GUANGDONG ACADEMY OF AGRI SCI
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