Insect antifeedant 15β-hydroxyklaineanone transported in plants and its application
An insect and plant technology, applied in the fields of plant growth regulators, applications, and repellents, can solve problems such as loss of efficacy and interference effects, and achieve the effects of environmental friendliness, high antifeedant activity, and convenient use.
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Embodiment 115
[0036]Example 115 The plant systemic properties of β-hydroxyklaineanone and its antifeedant activity after systemic absorption
[0037]Method: Dissolve 15β-hydroxyklaineanone with methanol, then prepare the solution to a final concentration of 100μg / mL, and spread it evenly on the surface of a 1.2 cm diameter circular cabbage leaf disc. The amount of medicine applied to each leaf disc When the methanol on the surface of the leaf disc is naturally dried, the 3rd instar larvae of Plutella xylostella (Linnaeus) are inserted. The treatments at each concentration are repeated 3 times at a relative humidity of 50% to 70% and a temperature of 25°C to 28 After keeping in an environment of ℃ for 24 hours, the feeding area was counted, and the feeding rate and refusal rate were calculated.
[0038]The antifeedant activity of 15β-hydroxyklaineanone on the first instar larvae of Spodopteralitura (Fabricius) was tested with cassava leaves; the antifeedant activity of 15β-hydroxyklaineanone on the firs...
Embodiment 2
[0046]Example 2 15β-hydroxyklaineanone can be absorbed into the roots of Shanghai greens from the growing environment of Shanghai greens through the roots, and is transported upwards to the leaves, so that insects refuse to eat greens green leaves
[0047]Method: Dissolve 15β-hydroxyklaineanone in DMSO to prepare a mother liquor of 10000μg / mL, then use 0.5mM CaCl2The aqueous solution dilutes the mother liquor to a 100μg / mL culture solution. Place the pre-cultivated Shanghai green seedlings (6 leaf stage, not treated with 15β-hydroxyklaineanone) in 0.5mM CaCl2After pre-incubating in an aqueous solution for 2 hours, transfer it to the above-mentioned prepared 15β-hydroxyklaineanone culture solution. After 24 hours, take 0.5 g of the apical leaves of the seaweed, mash it and dissolve it in 2 mL of methanol. The liquid chromatography-mass spectrometer can be used to It was detected that the leaf extract contained 15β-hydroxyklaineanone. According to calculations, the content of 15β-hydroxy...
Embodiment 3
[0049]Example 3: 15β-hydroxyklaineanone can be absorbed from the growth environment of the Chinese cabbage through the roots into the inside of the Chinese cabbage root, and transported upwards to the leaves, so that the vegetable aphid Lipaphiserysimi can resist eating the cabbage leaves
[0050]Method: Dissolve 15β-hydroxyklaineanone in methanol to prepare a mother liquor of 10000μg / mL, then use 0.5mM CaCl2The aqueous solution dilutes the mother liquor to a 100μg / mL culture solution. Place the previously cultivated cabbage seedlings (6 leaf stage, not treated with 15β-hydroxyklaineanone) in 0.5mM CaCl2Pre-incubate in aqueous solution for 2 hours and then transfer to the above prepared 15β-hydroxyklaineanone culture medium. After 24 hours, insert wingless cabbage aphids, 100 per cage, repeat 4 times, and count the control aphid 24 hours later. The calculation formula for the number of aphids living on the leaves and the treated leaves, and the antifeedant rate is as follows:
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