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Insect antifeedant eurycolactone F conducted in plants and its application

A plant and insect technology, applied in the fields of plant growth regulators, applications, and repellents, can solve the problems of losing efficacy and being easily affected by the interference of the external environment, and achieves high antifeedant activity and convenient use.

Active Publication Date: 2020-10-09
POMOLOGY RES INST GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, some compounds have been found to have antifeedant activity against insects, which are generally applied on the surface of plants to exert antifeedant activity, but this type of antifeedant needs to be sprayed, and it is easily affected by the interference of the external environment after application, such as rainwater washing will lead to loss of efficacy

Method used

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  • Insect antifeedant eurycolactone F conducted in plants and its application
  • Insect antifeedant eurycolactone F conducted in plants and its application
  • Insect antifeedant eurycolactone F conducted in plants and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 eurycolactone F can be sucked into the root of Chinese cabbage from the growth environment of Chinese cabbage through the root, and transported upward to the leaves, so that insects refuse to eat the leaves of Chinese cabbage

[0027] Method: Dissolve eurycolactone F in DMSO to make a mother solution of 10000μg / mL, and then use 0.5mM CaCl 2 Dilute the stock solution with aqueous solution to 100 μg / mL culture solution. The previously cultivated untreated Chinese cabbage seedlings (6-leaf stage) were placed in 0.5mM CaCl 2 After pre-cultivation in aqueous solution for 2 hours, transfer it into the 100 μg / mL eurycolactone F culture solution prepared above. After 24 hours of cultivation, take 0.55 g of the top leaf of Chinese cabbage, mash it, dissolve it in 2 mL of methanol, and use liquid chromatography- Mass spectrometry can detect that the extract contains eurycolactone F. After calculation, the content of eurycolactone F in the top leaves (fresh leaves) of ...

Embodiment 2

[0033] Example 2: Detecting the antifeedant activity of eurycolactone F to several leaf-eating Lepidoptera larvae that damage vegetables by the method of drug loading on leaf discs

[0034] Method: Dissolve eurycolactone in methanol to prepare a 2000 μg / mL mother solution, then dilute the mother solution with acetone to 5, 10 and 20 μg / mL solutions and spread them evenly on the surface of a circular cabbage leaf dish with a diameter of 1.5 cm. The amount of drug applied to each leaf dish was 20 μL. After the acetone on the surface of the leaf dish was naturally dried, the 2nd instar larvae of the diamondback moth Plutellaxylostella (Linnaeus) were inserted, and each concentration treatment was repeated 3 times. After keeping in an environment with a temperature of 25° C. to 28° C. for 24 hours, the feeding area was counted, and the feeding rate and refusal rate were calculated. See Example 1 for the calculation formula.

[0035] Cassava leaves were used to test the antifeedant...

Embodiment 3

[0039] Example 3: eurycolactone F can be sucked into the root of Chinese cabbage from the growth environment of Chinese cabbage through the root, and transported upward to the leaves, so that the cabbage aphid Lipaphiserysimi refuses to eat the leaves of Chinese cabbage

[0040] Experimental method: Dissolve eurycolactone F in methanol to make a mother solution of 10000μg / mL, and then use 0.5mMCaCl 2 Dilute the stock solution with aqueous solution to 100 μg / mL culture solution. The previously cultivated cabbage seedlings (6-leaf stage, not treated with eurycolactone F before) were placed in 0.5mM CaCl 2 After pre-cultivation in aqueous solution for 2 hours, transfer to the above-mentioned eurycolactone F culture solution that has been prepared. After 24 hours, insert the aphid without wings, insert 100 heads in each cage, repeat 4 times, and count the control leaves after 24 hours after inserting the aphid. and the number of cabbage aphids perched on the treated leaves, the f...

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Abstract

The invention discloses an insect anti-feedant eurycolactone F conducted in plants and an application thereof, i.e. eurycolactone F. The eurycolactone F has anti-feeding activity on various insects and can be conducted in plants; anti-feeding effect is better that anti-feedants applied to surface of plants. The eurycolactone F can be used for preparing systemic translocation insect anti-feedants and is more convenient to use, and is not liable to be interfered and influenced by external environment. The compound is prepared from natural plants through separation and purification and is low-toxicity, residual-free and pollution-free on economic crops such as cereal, melons and fruits, vegetable and the like. The compound has high anti-feeding activity on insects and has effect of preventionand treatment on pests.

Description

technical field [0001] The invention belongs to the fields of biological pesticides and plant protection, and in particular relates to an insect repellant eurycolactone F transported in plants and its application. Background technique [0002] The harm of agricultural pests has always been a serious problem in agricultural planting. In today's society, chemical pesticide control is still the main measure. Due to the long-term use of chemical pesticides for prevention and control, the pollution of land, water, and air, the emergence of plant resistance, and the significant decline in the control effect have forced farmers to increase the amount of pesticides used to improve the control effect. It makes pest control work difficult, and also causes more serious environmental problems and crop safety problems. Therefore, it is particularly important to explore efficient, safe and environment-friendly green pesticides. The development of botanical pesticides has always been a r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/12A01P17/00
CPCA01N43/12
Inventor 邵雪花赵天艺匡石滋赖多肖维强王晓容
Owner POMOLOGY RES INST GUANGDONG ACADEMY OF AGRI SCI
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