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Rhododendron oreodoxa Franch seed tissue culture speed propagation method

A technology of tissue culture rapid propagation and Rhododendron, which is applied in horticultural methods, botanical equipment and methods, culture medium, etc., can solve the problems of slow propagation speed, great influence of mother plant material and breeding season, etc., and achieve fast multiplication speed, Promote the healthy and rapid growth of root system and shorten the effect of multiplication time

Pending Publication Date: 2019-03-22
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the above-mentioned deficiencies in the prior art, the present invention provides a method for tissue culture and rapid propagation of rhododendron seeds, which can effectively solve the problem of being greatly affected by the mother plant material and the breeding season and slow in the propagation speed in the existing propagation methods. question

Method used

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  • Rhododendron oreodoxa Franch seed tissue culture speed propagation method
  • Rhododendron oreodoxa Franch seed tissue culture speed propagation method
  • Rhododendron oreodoxa Franch seed tissue culture speed propagation method

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Effect test

Embodiment 1

[0028] A kind of Shanguang rhododendron tissue culture rapid propagation method is characterized in that, comprises the following steps:

[0029] (1) Material collection and sterilization: select mature Rhododendron shanguang seeds, wrap them in gauze, wash them under running water for 10 minutes, put them on an ultra-clean workbench, rinse them with 75% alcohol for 30 seconds, and then wash them twice with sterile water , then soak and disinfect with 2% sodium hypochlorite for 2 minutes, and finally rinse with sterile water for 3 times;

[0030] (2) medium culture: the seeds after step (1) are sown in the Anderson medium containing the sucrose of 28g / L and the agar of 4g / L, the pH value of medium is 5.8, at temperature (25 ± 2 )°C, the relative air humidity is about 60%, the light time is 16h / d, and the light intensity is 4000-5000lx, and the germination rate, germination potential and germination time lag are counted;

[0031] (3) Proliferation culture: Cut 1-1.5cm stem seg...

Embodiment 2

[0036] A kind of Shanguang rhododendron tissue culture rapid propagation method is characterized in that, comprises the following steps:

[0037] (1) Material collection and sterilization: select mature Rhododendron shanguang seeds, wrap them in gauze, wash them under running water for 10 minutes, put them on an ultra-clean workbench, rinse them with 75% alcohol for 30 seconds, and then wash them twice with sterile water , then soak and disinfect with 2% sodium hypochlorite for 4 minutes, and finally rinse with sterile water for 3 times;

[0038] (2) medium culture: the seeds after step (1) are sown in the Anderson medium containing the sucrose of 32g / L and the agar of 8g / L, the pH value of medium is 5.8, at temperature (25 ± 2 )°C, the relative air humidity is about 60%, the light time is 16h / d, and the light intensity is 4000-5000lx, and the germination rate, germination potential and germination time lag are counted;

[0039] (3) Proliferation culture: cut 1-1.5cm stem seg...

Embodiment 3

[0044] A kind of Shanguang rhododendron tissue culture rapid propagation method is characterized in that, comprises the following steps:

[0045] (1) Material collection and sterilization: select mature Rhododendron shanguang seeds, wrap them in gauze, wash them under running water for 10 minutes, put them on an ultra-clean workbench, rinse them with 75% alcohol for 30 seconds, and then wash them twice with sterile water , then soak and disinfect with 2% sodium hypochlorite for 3 minutes, and finally rinse with sterile water for 3 times;

[0046] (2) medium culture: the seeds after step (1) are sown in the Anderson medium containing the sucrose of 30g / L and the agar of 6g / L, the pH value of medium is 5.8, at temperature (25 ± 2 )°C, the relative air humidity is about 60%, the light time is 16h / d, and the light intensity is 4000-5000lx, and the germination rate, germination potential and germination time lag are counted;

[0047] (3) Proliferation culture: cut 1-1.5cm stem seg...

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Abstract

The invention discloses a rhododendron oreodoxa Franch seed tissue culture speed propagation method which comprises the following steps of: material taking and sterilization, i.e., selecting mature seeds of rhododendron oreodoxa Franch and carrying out sterilization and disinfection for later use; culture of a culture medium, i.e., sowing the processed seeds in an Anderson culture medium to culture; multiplication culture, i.e., shearing 1 to 1.5cm of stems from aseptic seedlings and inoculating the stems into a multiplication culture medium to culture; test-tube rooting, i.e., when cluster buds obtained by multiplication culture grow to 2cm, placing the cluster buds in a culture flask containing a rooting culture medium to carry out rooting culture; exercising seedlings, i.e., when root systems of tissue culture seedlings grow to 1 to 2cm, carrying out seedling exercising; transplantation, i.e., placing roots of the tissue culture seedlings into carbendazim solution to soak, and finally, transplanting the tissue culture seedlings into a mixed medium. The method can effectively solve problems of large influence caused by a stock plant material and the breeding season and low breeding speed of an existing breeding method.

Description

technical field [0001] The invention relates to the technical field of rhododendron propagation, in particular to a method for tissue culture and rapid propagation of Rhododendron Shanguang seeds. Background technique [0002] Rhododendron resources are very rich in our country, but the development and utilization of Rhododendron are very limited. Wild rhododendron resources have been destroyed by human beings and illegal harvesting is common, and the number of some important wild rhododendron resources has decreased sharply or is on the verge of extinction. Therefore, it is very urgent to protect, develop and utilize wild Rhododendron resources. Shanguang Rhododendron is distributed in southern Gansu, western Hubei, and western to northwestern Sichuan. It grows in forests or miscellaneous forests and arrow bamboo bushes at an altitude of 2100-3650 meters. cause havoc. Commonly adopted propagation methods are sowing, cutting, grafting, etc. These propagation methods are a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G24/28A01G24/15
CPCA01H4/001A01H4/008A01G24/15A01G24/28
Inventor 姜贝贝潘远智黄文沛牟津阳李雨萌黄曼施刘庆林郑思聪廖菲娅蒋佳慧白丽蓉
Owner SICHUAN AGRI UNIV
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