Rapid propagation method for tissue culture of rubus sachalinensis adventitious buds

A technology for the proliferation and culture of Rubus sclerotiorum is applied in the field of rapid propagation of tissue culture of adventitious buds of Rubus sclerotiorum, which can solve problems such as lack of resources, and achieve the effects of protecting the ecological environment, protecting the environment and high survival rate.

Inactive Publication Date: 2019-03-22
INNER MONGOLIA MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The rapid traditional tissue technology can provide an effective way to solve the shortage of its resources. At present, there are no reports on the rapid propagation of Rubus sakhalen and its wild rearing in China.

Method used

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  • Rapid propagation method for tissue culture of rubus sachalinensis adventitious buds
  • Rapid propagation method for tissue culture of rubus sachalinensis adventitious buds
  • Rapid propagation method for tissue culture of rubus sachalinensis adventitious buds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Embodiment 1, Rapid Propagation Method of Adventitious Bud Tissue Culture of Rubus kuye

[0057] 1. Selection of explants

[0058] In May and September 2017, at the Sumushan Forest Farm in Xinghe County, Ulanqab City, Inner Mongolia Autonomous Region, a robust and pest-free Rubus sakhalin daughter plant was selected, and the adventitious buds were removed completely, and stored at 4°C with moisture .

[0059] 2. Disinfection of explants

[0060] Peel off the outer scales of the adventitious buds obtained in step 1, wash twice with a concentration of 1% detergent (Guangzhou Liby Enterprise Group Co., Ltd., catalog number 6920174736939), and rinse with sterile water for 2 hours. On the ultra-clean workbench, soak for 30s with 75% ethanol solution, and then use 0.1% HgCl 2 The solution (adding 0.5-1 drop of Tween 80) was sterilized for 8 minutes, washed with sterile water for 5 times, and the sterilized explants were obtained.

[0061] 3. Proliferation of adventitious ...

Embodiment 2

[0068] Embodiment 2, Rapid Propagation Method of Adventitious Bud Tissue Culture of Rubus kuye

[0069] 1. Selection of explants

[0070] In May and September 2017, at the Sumushan Forest Farm in Xinghe County, Ulanqab City, Inner Mongolia Autonomous Region, a robust and pest-free Rubus sakhalin daughter plant was selected, and the adventitious buds were removed completely, and stored at 4°C with moisture .

[0071] 2. Disinfection of explants

[0072] Peel off the outer scales of the adventitious buds obtained in step 1, wash twice with 1% detergent, and rinse with sterile water for 2.5 hours. On the ultra-clean workbench, soak for 25s with 75% ethanol solution, and then use 0.1% HgCl 2 The solution (adding 0.5-1 drop of Tween 80) was sterilized for 7 minutes, washed with sterile water for 3 times, and the sterilized explants were obtained.

[0073] 3. Proliferation of adventitious buds

[0074] Peel off the last layer of scales from the sterilized explants in step 2, cu...

Embodiment 3

[0080] Embodiment 3, Rapid Propagation Method of Adventitious Bud Tissue Culture of Rubus kuye

[0081] 1. Selection of explants

[0082] In May and September 2017, at the Sumushan Forest Farm in Xinghe County, Ulanqab City, Inner Mongolia Autonomous Region, a robust and pest-free Rubus sakhalin daughter plant was selected, and the adventitious buds were removed completely, and stored at 4°C with moisture .

[0083] 2. Disinfection of explants

[0084] Peel off the outer scales of the adventitious buds obtained in step 1, wash twice with 1% detergent, and rinse with sterile water for 2 hours. On the ultra-clean workbench, soak for 25s with 75% ethanol solution, and then use 0.1% HgCl 2 The solution (adding 0.5-1 drop of Tween 80) was sterilized for 6 minutes, washed with sterile water for 4 times, and the sterilized explants were obtained.

[0085] 3. Proliferation of adventitious buds

[0086] Peel off the last layer of scales from the sterilized explants in step 2, cut ...

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Abstract

The invention discloses a rapid propagation method for tissue culture of rubus sachalinensis adventitious buds. According to the invention, rubus sachalinensis adventitious buds are selected as explants, and rubus sachalinensis test-tube plantlets are finally acquired through the processes of disinfection, inductive proliferation and rooting induction of the explants. The rapid propagation methodhas the advantages of rapid propagation, high rate of survival, environmental protection and being not influenced by season, soil, moisture, and the like. The invention not only provides a new methodfor tissue rapid propagation technology of rubus sachalinensis but also establishes a basis for manual large-scale planting of rubus sachalinensis and has significance in conserving, developing and utilizing genetic resource of rubus sachalinensis.

Description

technical field [0001] The invention relates to the technical field of plant breeding, in particular to a rapid propagation method for tissue culture of adventitious buds of Rubus khalien. Background technique [0002] Rubus sachalinensis Lévl. is a shrub of the genus Rubus in the Rosaceae family. It grows in dense forests, forest meadows or crevices of dry ditches on hillsides at an altitude of 1000-2500 meters. It is distributed in my country. Inner Mongolia, Heilongjiang, Jilin, Hebei, Gansu and other places. [0003] The stems, leaves, roots and fruits of Rubus kuye can be used as medicine: the stems and leaves have the effects of hemostasis, detoxification, anti-inflammatory, and rheumatism; Among them, the dried stem of this product is one of the commonly used Mongolian medicinal materials. In the "Drug Standards of the Ministry of Health of the People's Republic of China Mongolian Medicine Sub-volume", it is mainly used to treat colds, coughs, immature fever, "Hey" f...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008
Inventor 包保全岳鑫王艳丽李桂林王晓琴张英白雪飞
Owner INNER MONGOLIA MEDICAL UNIV
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