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Diagnosis and treatment application of mononuclear/phagocyte cells to aortic injuries

A macrophage, aortic dissection technology, applied in the field of biomedicine, can solve the problem of low incidence of AD

Inactive Publication Date: 2019-03-19
SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This type of model is more likely to form aortic aneurysm, and the incidence of AD is lower, so there is an urgent need for an animal model that can reflect the tissue changes before the occurrence of dissection and can stably form dissection

Method used

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  • Diagnosis and treatment application of mononuclear/phagocyte cells to aortic injuries
  • Diagnosis and treatment application of mononuclear/phagocyte cells to aortic injuries
  • Diagnosis and treatment application of mononuclear/phagocyte cells to aortic injuries

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0184] Infiltration of inflammatory cells in the aorta during the course of AD

[0185] 1.1 Experimental materials

[0186] experimental animals

[0187] C57 mice: 64 males, provided by the Shanghai Experimental Animal Center of the Chinese Academy of Sciences, were raised in the SPF animal room of the Experimental Animal Center of the Shanghai Institute of Materia Medica, the Chinese Academy of Sciences, with a constant temperature of 22±2°C, 12h of light, a standard diet, and free drinking water.

[0188] High-fat feed: the feed contains 18.8% protein and 16.2% fat, provided by Shanghai Slack Experimental Animal Co., Ltd.

[0189] 1.2 Experimental method

[0190] 1.2.1 Preparation of aortic dissection model

[0191] In order to establish a mouse model simulating the AD disease process, C57 mice were randomly divided into 4 groups according to body weight: 0% β-aminopropionitrile group (n=16), 0.125% β-aminopropionitrile group (n=16) , 0.25% β-aminopropionitrile group (n=...

Embodiment 2

[0232] Example 2 Infiltration of inflammatory cells before and after acute aortic dissection

[0233] 2.1 Experimental materials

[0234] C57 mice: 42 males, provided by Shanghai Experimental Animal Center, Chinese Academy of Sciences

[0235] 2.2 Experimental method

[0236] 2.2.1 Preparation of acute aortic dissection model

[0237] In order to further study the role of inflammatory cells in the occurrence of AD, we established a research system for acute AD ( Figure 12 ), the time point of AD occurrence was defined as the time from the aortic injury stage before dissection (simulated by β-aminopropionitrile) to aortic tear or rupture (simulated by β-aminopropionitrile+AngII) Conversion, and its role was further explored by comparing the changes of inflammatory cells in aortic tissue at these two time points. 42 C57BL / 6 mice, all male, weighing 11±2g, were raised in the SPF animal room of the Experimental Animal Center of Shanghai Institute of Materia Medica, Chinese Ac...

Embodiment 3

[0251] Example 3 Research on the role of T lymphocytes in the occurrence of aortic dissection

[0252] Previous experiments have clarified the infiltration of inflammatory cells in the aortic injury site, but the role of these inflammatory cell infiltration in the aorta in the process of the disease has not been clarified. Nude mice (nu / nu mice) are congenitally deficient in the FOXN1 gene, resulting in a deficiency in the immune system of T lymphocytes. This example uses nude mice to study the impact of T lymphocyte deficiency on the occurrence of aortic dissection.

[0253] 3.1. Experimental materials

[0254] 3.1.1 Experimental animals

[0255] BALB / c mice: 36 males, provided by Shanghai Experimental Animal Center, Chinese Academy of Sciences

[0256] Nu / nu mice: male, 36, provided by Shanghai Experimental Animal Center, Chinese Academy of Sciences

[0257] 3.2 Preparation of acute aortic dissection model

[0258] 36 BALB / c mice, all male, weighing 10±2g, were raised in...

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Abstract

The invention discloses diagnosis and treatment application of mononuclear / phagocyte cells to aortic injuries. Particularly, aortic dissection (AD) occurs along with infiltration of mononuclear / phagocyte cells at aortic injury parts, so that aortic dissection (AD) related diseases (such as vascular tear, vascular wall thickening, thrombi formed between the tunica media and the adventitia and elastic fiber breakage) can be diagnosed according to infiltration conditions of the mononuclear / phagocyte cells. In addition, the invention further discloses a fact that mononuclear / phagocyte cell knockout can remarkably prevent and / or treat aortic dissection (AD) related diseases and discloses a mononuclear / phagocyte cell inhibitor for remarkable prevention and / or treatment of aortic dissection (AD)related diseases.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the diagnosis and treatment application of monocyte / macrophage in aortic injury. Background technique [0002] AD is a very dangerous aortic disease with the characteristics of acute onset, rapid progression and high mortality. In recent years, with the aging of the population, the incidence of hypertension and atherosclerosis has increased, and the incidence of AD has also shown a gradually increasing trend. Since the pathogenesis of AD is still unclear, the clinical prevention and treatment of this disease is seriously restricted. [0003] At present, the tissues used for clinical AD research are often obtained during the patient's operation, that is to say, only the patient samples after the onset of the disease can be obtained. Since the occurrence of AD cannot be predicted, it is also impossible to obtain the patient samples before the onset of the disease, so the clinical sample...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569A61K45/00A61K39/395A61P9/00
CPCA61K45/00G01N33/56966A61K2039/505A61K39/395A61P9/00G01N33/00G01N33/569G01N33/68
Inventor 姜宝红王琳琳
Owner SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
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