Molecular marker primer composition for identifying inter-hybrid seeds of pears and apples and application thereof
A primer composition and molecular marker technology, applied in the field of molecular genetics and breeding, can solve the problems of less polymorphic sites, long waiting time, cumbersome and time-consuming operation, etc., and achieve improved accuracy and efficiency, stable and reliable results, and specificity. good effect
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[0027] Molecular markers for the identification of hybrids between pear and apple genera based on genome-specific sequences were obtained by the following methods:
[0028] a) Using 'Hongxing'×'Coomice', 'Jinguan'×'Dangshansuli', 'Fuji'×'Coomice' for intergeneric hybridization, 73, 37 and 201 plants were obtained respectively—a total of 311 F1 offspring of apple and pear plants.
[0029]b) The DNA extracted based on the CTAB method has a higher concentration and better quality, and the operation steps for extracting DNA are as follows:
[0030] (1) Collect the disease-free and clean young leaves of the parents and hybrid offspring in April in spring, store them in ziplock bags, and freeze them quickly in liquid nitrogen. Take 100mg leaves, add liquid nitrogen to the mortar and grind them thoroughly; then transfer the ground leaves to a 1.5ml centrifuge tube, add 400μl CTAB buffer, mix well and put them in a 65°C water bath for 30min, every Shake once every 10 minutes to full...
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