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Preparation method of temporary slice of mature corn root system

A technology for slicing and corn, which is applied in the field of plant root slice preparation, can solve problems such as unclear images and observation errors, achieve clear observation images, reduce observation errors, and save time

Inactive Publication Date: 2019-03-12
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Aiming at the problem that the image is not clear in the preparation process of the existing temporary slice, so as to produce the problem of observation error, the invention provides a kind of preparation method of the temporary slice of mature corn root system, comprising the following steps:

Method used

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  • Preparation method of temporary slice of mature corn root system
  • Preparation method of temporary slice of mature corn root system

Examples

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Comparison scheme
Effect test

Embodiment 1

[0024] Example 1. Preparation method of temporary slices of mature corn root system.

[0025] 1) Wash the retrieved corn root material at the milk maturity stage with distilled water repeatedly, flatten the cross-section of the material, cut into 1-2cm pieces, and fix it in FAA fixative (standard fixative) for 24h.

[0026] 2) After taking out the material, use a double-sided blade to make the blade slide steadily with even force. Cut off the slice and gently move it into a petri dish filled with water.

[0027] 3) Choose thin and transparent sections with a thickness of 50-100 μm, stain them with 1% safranin aqueous solution for 30 seconds, and place them in the center of the slide. 30s is the best dyeing time, too long will easily cause excessive dyeing and affect image observation.

[0028] 4) Drip 3-4 drops of double distilled water, 120μL is suitable on the slice, then put the slice into the vacuum pump, under the condition of 8torr vacuum, vacuum for 7min (until the slice and th...

Embodiment 2

[0029] Example 2. Preparation method of temporary slices of mature corn root system.

[0030] 1) Wash the retrieved corn root material at the milk maturity stage with distilled water repeatedly, flatten the cross-section of the material, cut into pieces of 1-2 cm, and fix it in FAA fixative (standard fixative) for 20 hours.

[0031] 2) After taking out the material, use a double-sided blade to make the blade slide steadily with even force. Cut off the slice and gently move it into a petri dish filled with water.

[0032] 3) Select thin and transparent sections with a thickness of 50-100 μm, stain them with 1% safranin aqueous solution for 20 seconds, and then place them in the center of the glass slide.

[0033] 4) Drop 3-4 drops of double-distilled water, 150μL is suitable on the slice, then put the slice into the vacuum pump, under the condition of 5torr vacuum, vacuum for 5min (until the slice and the slide are completely attached), take it out Cover with a cover glass and gently p...

Embodiment 3

[0034] Example 3. Preparation method of temporary slices of mature corn root system.

[0035] 1) Wash the retrieved corn root material at the milk maturity stage repeatedly with distilled water, flatten the cross-section of the material, cut it into 1-2cm pieces, and fix it in Carnot's solution for 72 hours.

[0036] 2) After taking out the material, use a double-sided blade to make the blade slide steadily with even force. Cut off the slice and gently move it into a petri dish filled with water.

[0037] 3) Pick a thin and transparent section with a thickness of 50-100μm, stain it with a 1% safranin aqueous solution for 40s, and then place it in the center of the glass slide.

[0038] 4) Drop 3-4 drops of double-distilled water, 130μL is suitable on the slice, then put the slice into the vacuum pump, under the condition of 10torr vacuum, vacuum for 10min (until the slice and the slide are completely attached), take it out Cover with a cover glass and gently press the cover glass with...

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Abstract

The invention discloses a preparation method of a temporary slice of a mature corn root system, belonging to the technical field of preparation of slices of plant root systems. Aiming at the problemsin the existing temporary slice preparation process that an image is unclear and leading to observational error, the invention provides the preparation method of the temporary slice of the mature cornroot system, which is characterized in that a dyed slice is subjected to vacuumizing treatment so as to remove 99% of bubbles in the slice, so that an observation image is more clear, error is reduced, and convenience is provided for research on anatomical structures of the mature corn root system.

Description

Technical field [0001] The invention belongs to the technical field of plant root system slice preparation, and specifically relates to a preparation method of mature corn root system temporary slices. Background technique [0002] Temporary slicing is used to observe the internal structure of plants, but in actual experiments, it is found that the traditional method of temporary slicing is used for relatively tender plant tissues and has better results. However, when the plant enters the mature stage, especially the corn root system, the cell wall becomes thicker , After slicing, there will be a lot of bubbles during observation, which will affect the observation results. Summary of the invention [0003] Aiming at the problem that the image is not clear during the preparation process of the existing temporary slices to cause observation errors, the present invention provides a preparation method of the temporary slices of mature corn root system, which includes the following ste...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/06G01N1/30G01N1/28
CPCG01N1/06G01N1/28G01N1/286G01N1/30G01N2001/2873
Inventor 王彬李彩凤刘磊佟桐刘笑鸣赵猛陈婧婷刘新宇张树友刘丹邹春雷武沛然杨芳芳王玉波
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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