Rotator cuff bioremediation mesh patch as well as use and preparation method thereof
A rotator cuff and biological technology, applied in the field of rotator cuff biological repair mesh and its use and preparation, can solve the problems of tissue regeneration mismatch, cytotoxicity, slow degradation rate, etc., to reduce the incidence of inflammation and infection, and accelerate Effect of functional reconstruction, good mechanical properties
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Embodiment 1
[0056] Preparation of Porcine Small Intestinal Submucosal Rotator Cuff Bioprosthetic Mesh, see Figure 5 :
[0057] (1) Pretreatment
[0058] Freshly slaughtered porcine small intestine tissues were cleaned, soaked in 0.5% acetic acid solution for 30 minutes, the ratio of porcine small intestine to acetic acid solution was 1:5, and the mucosal layer, muscular layer and serosa layer of porcine small intestine and jejunum were removed by physical scraping , lymph nodes, the submucosa was separated, cut into thin strips evenly in the longitudinal direction, and washed 3 times with purified water to obtain the rotator cuff biological repair material, that is, the small intestinal submucosa, hereinafter referred to as SIS material.
[0059] (2) Virus inactivation
[0060] Use a mixed aqueous solution containing 1.0% peracetic acid and 15% ethanol, the ratio of the SIS material to the mixed aqueous solution is 1:10, and soak at room temperature for 100 minutes under ultrasonic con...
Embodiment 2
[0071] Preparation of bio-mesh for rotator cuff repair in porcine small intestine submucosa
[0072] (1) Pretreatment
[0073] Freshly slaughtered pig small intestine tissues were cleaned and soaked in 0.01% acetic acid solution for 120 minutes. The ratio of pig small intestine to acetic acid solution was 1:10. The mucosal layer, muscular layer and serosa layer of pig small intestine jejunum were removed by physical scraping , Lymph nodes, the submucosa was separated, cut into pieces, and washed 3 times with purified water.
[0074] (2) Virus inactivation
[0075] Use a mixed aqueous solution containing 0.5% peracetic acid and 25% ethanol, the ratio of the SIS material to the mixed aqueous solution is 1:15, and soak at room temperature for 120 minutes under ultrasonic conditions to inactivate the virus. Afterwards, it was ultrasonically washed 3 times with purified water.
[0076] (3) Degreasing
[0077] Use ethanol with a concentration of 90%, the ratio of SIS material to...
Embodiment 3
[0086] For the safety of the samples, the samples prepared in Example 1-2 were tested for immunogenic substances.
[0087] (1) Detection method of residual cells: fixed with 10% neutral formalin, embedded in paraffin, cut into thin slices of 0.4 micron, dewaxed with xylene, dehydrated with serial alcohol, stained with hematoxylin-eosin, and microscope Next, observe the cell residue and matrix fiber structure.
[0088] (2) Detection method of DNA content: according to YY / T 0606.25-2014 "Method for determination of DNA residues in biological materials of animal origin: fluorescent staining method".
[0089] (3) Detection method of α-Gal antigen content: After the sample was fixed with paraformaldehyde, it was routinely embedded in paraffin and sectioned, with a thickness of 3 μm. Immunohistochemical reaction was carried out by using the specific affinity between biotin-labeled BSI-B4 and α-Gal antigen. Judgment of staining results: dark brown-yellow particles are strongly posi...
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