Application of chrysanthemum morifolium ramat. steam leaf extract as bacteriostatic agent and preparation method of chrysanthemum morifolium ramat. steam leaf extract
The invention relates to a technology for the extraction of chrysanthemum chrysanthemum, which is applied in the application and preparation field of the stem and leaf extract of chrysanthemum chrysanthemum as a bacteriostatic agent, and can solve the problems of environmental pollution, very little research on the extraction of antibacterial components and their application, waste of natural resources, and the like, Achieve obvious bacteriostatic activity, enrich comprehensive utilization channels, and improve the economic value of industrialization
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[0025] Embodiment 1: Preparation of Chrysanthemum chrysanthemum stem and leaf extract
[0026] (1) Pre-treatment: Clean the stem and leaf samples of Chrysanthemum chrysanthemum, dry them in an oven at 55°C to constant weight, crush them with a pulverizer, and pass them through a 100-mesh sieve to obtain medicinal material powder. Store the medicinal powder in a blue bottle for future use.
[0027] (2) Extraction: Weigh 10g of the dried medicinal material powder obtained in step (1), add 100mL of ethanol with a volume percentage of 75% (i.e., the ratio of solid to liquid is 1:10), soak overnight, reflux in a water bath at 90°C for 4h, and suction filter . Obtain medicinal material filtrate and medicinal residue.
[0028] (3) The medicinal residues were repeatedly extracted three times according to the conditions in step (2).
[0029] (4) Combine the medicinal material filtrate obtained in steps (2) and (3). After spinning off the ethanol solvent in the medicinal material fi...
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