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Rapid anti-double-stranded DNA (deoxyribonucleic acid) antibody detection method

A detection method and antibody technology, applied in the field of rapid detection of anti-double-stranded DNA antibodies, can solve the problems of low detection accuracy of lupus erythematosus, complicated operation, long time consumption, etc. Effect

Inactive Publication Date: 2019-03-08
江苏齐耀生物科技有限公司
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  • Abstract
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  • Claims
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Problems solved by technology

[0006] The purpose of the present invention is to solve the problems of low detection accuracy, cumbersome operation, and long time-consuming in the detection of systemic lupus erythematosus, and provide a rapid detection of anti-double-stranded DNA antibody that is easy to operate, saves time, and has high detection accuracy method

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  • Rapid anti-double-stranded DNA (deoxyribonucleic acid) antibody detection method

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Embodiment Construction

[0016] The present invention will be described in detail below in conjunction with the accompanying drawings and specific examples.

[0017] The present invention is illustrated in conjunction with examples, and the required reagents and materials include: plasmid, redistilled water, buffer, restriction endonuclease, ethylenediaminetetraacetic acid, absolute ethanol, ethanol solution, biotinyl water-soluble hydrazine, pentyl Dialdehyde, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride solution, F1-Y030 microsphere solution, N-hydroxysuccinimide solution, NaOH solution, NH4Cl solution, Antibody to be labeled, PBS buffer, bovine serum albumin solution, streptavidin, goat anti-mouse immunoglobulin G, NC membrane, sample pad, absorbent paper, NaN3, PVC bottom plate, required equipment includes: centrifuge tube, Centrifuge, water bath box, electronic pH meter, fluorescence immunoassay analyzer, continuous dot film and gold spray all-in-one machine.

[0018] The steps of...

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Abstract

The invention discloses a rapid anti-double-stranded DNA (deoxyribonucleic acid) antibody detection method. A DNA extraction method includes the steps: biotin labeling of double-stranded DNA antigens;fluorescence labeling of mouse anti-human immune globulin G; test strip and sample diluent preparation; rapid and accurate detection by the aid of a centrifuge tube, a centrifugal machine, a water bath box, an electronic pH (potential of hydrogen) meter, a fluorescence immunoassay instrument, a continuous point film spraying integrated machine and corresponding reagents and materials. According to the method, detection is implemented by a test strip, time is short, streptavidin protein is fixed by biotin labeled plasmid double-stranded DNA, so that sensitivity is improved, the test strip andsample diluent can be prepared in advance, so that the test strip and sample diluent is conveniently stored and taken in using, and the rapid anti-double-stranded DNA antibody detection method is simple and convenient to operate and high in detection accuracy and saves time.

Description

technical field [0001] The invention relates to the field of rapid detection, in particular to a rapid detection method for anti-double-strand DNA antibodies. Background technique [0002] In today's social life, people attach great importance to physical health, but the threat of diseases to health has not diminished, especially autoimmune diseases. Many diseases have been defined as autoimmune diseases one after another. In order to curb autoimmune diseases that continue to endanger people Improve the health of the people, improve the level of medical care, improve people's health, and conduct a lot of research on the early diagnosis of autoimmune diseases, in order to obtain accurate and fast diagnosis methods. [0003] Autoimmune diseases are diseases caused by the damage of self-organization due to the immune response to self-antigens. The specific clinical manifestations are organ-specific autoimmune diseases and systemic autoimmune diseases, and systemic autoimmune di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/533G01N33/543
CPCG01N33/533G01N33/543
Inventor 柏锦程张振
Owner 江苏齐耀生物科技有限公司
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