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Molecular marker for detecting susceptibility of cervical cancer, kit and application

A molecular marker and susceptibility technology, applied in the fields of molecular biology and biomedicine, can solve problems such as increasing the risk of carcinogenesis, affecting protein function, and changing cell fate, achieving the effects of low cost, high specificity, and simple detection.

Active Publication Date: 2019-03-08
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the genetic variation of NEIL1 and NEIL2 may affect the protein function, lead to the change of cell fate and increase the risk of cancer, but there is no literature report that the SNP sites in these two genes are related to the susceptibility to cervical cancer

Method used

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  • Molecular marker for detecting susceptibility of cervical cancer, kit and application
  • Molecular marker for detecting susceptibility of cervical cancer, kit and application
  • Molecular marker for detecting susceptibility of cervical cancer, kit and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1. Association analysis of NEIL2 gene single nucleotide polymorphism detection and cervical cancer susceptibility

[0031] 1.1 Research object

[0032] 400 cases of cervical squamous cell carcinoma and 400 cases of cervical precancerous lesion CIN III were randomly selected. The selected cases were from June 2004 to December 2008, and were sourced from the pathology of Obstetrics and Gynecology Hospital, Zhejiang University School of Medicine. Cases with a clear diagnosis, and 1,200 female volunteers with normal physical examinations were selected as normal controls. The selection criteria for normal controls were no pathological cytological findings, no endometriosis, no gynecological tumors, and no other solid cancers or immunity. disease. All cases and normal control individuals are Han residents. Approximately 2ml of peripheral anticoagulant blood from patients and normal volunteers was drawn and stored frozen at -80°C for later use.

[0033] All subjects were a...

Embodiment 2

[0051] Example 2. NEIL2 gene single nucleotide polymorphism and cervical cancer susceptibility detection kit

[0052] 1. Based on the results of Example 1, we found that 501G in SEQ ID NO:1 (rs804270)> The base variation of C is significantly related to the pathogenesis of cervical squamous cell carcinoma and its precancerous lesion CIN III. Therefore, we designed specific primers based on the NEIL2 gene single nucleotide polymorphism to detect the patient’s peripheral blood DNA template Kit.

[0053] 2. Prepare a special kit (100tests), see Table 4 for specific reagent composition, as follows:

[0054] Table 4. Reagent composition of NEIL2 gene SNP site detection kit

[0055] Reagent name (concentration)

Total amount of kit

10×PCR Buffer(Mg 2+ 25mM)

200μL

dNTP Mixture (2.5mM each)

800μL

Taq DNA polymerase(5U / μL)

20μL

Upstream primer 1 (SEQ ID NO: 2) (100pmol)

100μL

Upstream primer 2 (SEQ ID NO: 3) (100pmol)

100μL

Upstream primer 3 (SEQ ID NO: 4) (100pmol)

100μL...

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Abstract

The invention discloses a molecular marker for detecting the susceptibility of a cervical cancer, a kit and application, and belongs to the technical field of molecular biology and biological medicines. The nucleotide sequence of the molecular marker is as shown in SEQ ID NO. 1, and single nucleotide polymorphism exists at a 501th position: G> C or G> T, namely, basic groups at the 501th positionof the sequence comprise a basic group G, a basic group C and a basic group T. The provided kit has three pairs of specific primers based on NEIL2 gene single nucleotide polymorphism design, NEIL2 gene single nucleotide polymorphism is closely related to the susceptibility of the cervical cancer, by a PCR technology, the single nucleotide polymorphism in NEIL2 gene can be detected effectively, then the susceptibility of the cervical cancer is identified, and thus, the molecular marker plays an important practical significance on screening of high risk groups of the cervical cancer and detection of susceptible individuals, and early prevention, early diagnosis and individualized prevention and treatment on the cervical cancer which is a complicated disease.

Description

Technical field [0001] The present invention relates to the technical field of molecular biology and biomedicine, in particular to a molecular marker for detecting the susceptibility of cervical cancer and its application. Background technique [0002] Cervical cancer is the fourth most common cancer among women worldwide, with an estimated 529,572 newly diagnosed cases and 274,967 deaths each year. At the same time, cervical cancer is also the third leading cause of cancer-related deaths in women worldwide, and the incidence has been increasing. In China, in addition to breast cancer, cervical cancer has become the most common cancer in women (98.9 per 100,000), with a mortality rate of 30.5 per 100,000, and the incidence rate is also increasing. [0003] High-risk human papillomavirus (HPV) is the main cause of cervical cancer. However, although about 80% of women may be infected with HPV in their lifetime, only a small percentage of them develop malignant tumors. According to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/156
Inventor 陈怀增叶枫王浛知洪蝶程琪周彩云
Owner ZHEJIANG UNIV
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