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Application of mesenchymal stem cell exosome in preparation of drug preparation for promoting hair regeneration

A technology of mesenchymal stem cells and pharmaceutical preparations, applied in the field of mesenchymal stem cell exosomes, to achieve the effect of promoting hair regeneration, simple method, and promoting transformation

Inactive Publication Date: 2019-03-08
海门生原干细胞科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are no literature reports on the application of exosomes in hair regeneration

Method used

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  • Application of mesenchymal stem cell exosome in preparation of drug preparation for promoting hair regeneration
  • Application of mesenchymal stem cell exosome in preparation of drug preparation for promoting hair regeneration
  • Application of mesenchymal stem cell exosome in preparation of drug preparation for promoting hair regeneration

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Experimental program
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Effect test

Embodiment 1

[0036]Isolation and identification of exosomes from mesenchymal stem cells

[0037] 1. Isolation, culture and identification of mesenchymal stem cells (MSCs)

[0038] The umbilical cord came from the Obstetrics and Gynecology Department of Changhai Hospital, and the mesenchymal stem cells were obtained from primary culture. After harvesting the umbilical cord, put it in a 50ml centrifuge tube containing sterile phosphate buffered saline PBS (Gibco, product number: C14190500BT) or low-sugar DMEM medium (Gibco, product number: 11885-084), and operate as soon as possible. After repeated washing with PBS, soak in low-sugar-DMEM (concentration of penicillin and streptomycin is 100IU / ml) containing penicillin and streptomycin (Gibco, product number: 15140-122) for about 5 minutes, and cut into diameters of about 1.5mm. Tissue blocks; low-sugar-DMEM nutrient solution containing 10% fetal bovine serum, 5% CO 2 , Culture at 37°C with saturated humidity; after 3-5 days, mesenchymal st...

Embodiment 2

[0050] The exosomes secreted by umbilical cord mesenchymal stem cells can improve the microenvironment of skin tissue in alopecia areata and promote hair regeneration. All animal experiments complied with the requirements of NIH experimental animals.

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Abstract

The invention discloses application of mesenchymal stem cell exosome to preparation of a drug preparation for promoting hair regeneration. Mesenchymal stem cells are prepared by the following method:separating and extracting the mesenchymal stem cells from an umbilical cord and then obtaining the mesenchymal stem cells through primary culture and subculture. The application of the mesenchymal stem cell exosome to preparation of the drug preparation for promoting the hair regeneration, disclosed by the invention, has the advantages that skin tissue structures on alopecia areata can be improvedby adopting the mesenchymal stem cell exosome, and hair follicles in the stationary phase in the skin tissue on the alopecia areata can be promoted to be transformed to a growing period; and the hairregeneration on the alopecia areata can be promoted. The application prospect of the mesenchymal stem cell exosome in preparation of the drug preparation for promoting the hair regeneration is broad.

Description

technical field [0001] The invention belongs to the technical field of application of exosomes of mesenchymal stem cells, and in particular relates to the application of exosomes of mesenchymal stem cells in the preparation of pharmaceutical preparations for promoting hair regeneration. Background technique [0002] There are many diseases that cause hair loss, the most common of which is alopecia areata. Alopecia areata, commonly known as "ghost shaved head", is a sudden localized patchy hair loss with normal local skin and no symptoms. The etiology is currently unknown, but pathological examination shows a decrease in hair follicles and lymphocyte infiltration around the hair follicles, and the disease is sometimes combined with other autoimmune diseases (Alopecia Areata: Review of Epidemiology, Clinical Features, Pathogenesis, and New Treatment Options. Darwin E, Hirt PA , Fertig R, Doliner B, Delcanto G, Jimenez JJ. Int J Trichology. 2018;10(2):51-60). Most common alop...

Claims

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Application Information

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IPC IPC(8): A61K35/28A61P17/14
CPCA61K35/28A61P17/14
Inventor 刘厚奇嵇铂尧
Owner 海门生原干细胞科技有限公司
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