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Simultaneous estrus-timed insemination treatment method for hybrid buffaloes

A treatment method and technology of timing insemination, applied in animal delivery, animal husbandry, veterinary instruments, etc., can solve the problems of unsatisfactory application effect, low conception rate, low estrus rate in the same period, and improve the estrus rate in the same period. The effect of breeding rate and conception rate, convenient operation and improving early embryo survival rate

Active Publication Date: 2019-03-08
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing estrus synchronization-timed insemination technology, such as GnRH-PG-GnRH (Ovsynch), is not very effective in hybrid buffalo, mainly manifested in low estrus synchronization rate and low conception rate.

Method used

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  • Simultaneous estrus-timed insemination treatment method for hybrid buffaloes
  • Simultaneous estrus-timed insemination treatment method for hybrid buffaloes
  • Simultaneous estrus-timed insemination treatment method for hybrid buffaloes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] In order to understand the influence of mifepristone injection dose on buffalo follicle development, estrus ovulation and conception, and improve the synchronous estrus effect of the synchronous estrus scheme, the present invention selects 120 healthy cows without reproductive tract diseases, and designs three meters Fepristone dose screening (0.3mg / kg, 0.4mg / kg, 0.5mg / kg), mifepristone injection time and the second injection of GnRH at the same time; at the same time, the well-recognized program GPG+CIDR ​​( vaginal suppository) as a control.

[0031] The specific operation is as follows:

[0032] In the afternoon of day 0, hybrid buffaloes were injected with GnRH intramuscularly at 400 μg / head. B-ultrasound examination was performed from day 6 (the day before PGF2ɑ injection) to ovulation on day 12 (that is, 72 hours after the second injection of GnRH), and follicular development was recorded. And estrus, ovulation status. In the afternoon of the 7th day, intramuscu...

Embodiment 2

[0039]For screening optimal mifepristone administration time, selected 52, healthy cows without reproductive tract disease, the present invention has designed 3 injection time points of injecting mifepristone, promptly the second injection GnRH injects simultaneously Mifepristone, 4 hours earlier or later than the second GnRH injection. The experimental operation was the same as that in Example 1, except that the second injection of GnRH was simultaneously injected with mifepristone, 4 hours earlier or later than the second injection of GnRH. The impact of different administration times of mifepristone on the effect of synchronous estrus is shown in Table 2. The results show that the follicular cyst rate and anestrus rate of the test group injected with GnRH at the same time as the second injection of GnRH are less than those of other test groups. Sexual estrus rate, total estrus rate, ovulation rate and conception rate were higher than other test groups (P>0.05). Four hours ...

Embodiment 3

[0045] In order to screen the best artificial insemination time, 100 healthy cows without reproductive tract diseases were selected, and the artificial insemination time was screened and optimized in this experiment. The timing of single and double timing insemination was designed. Three points were designed for the timing of single timing (20, 24, and 28 hours after the second GnRH injection), and the timing of double timing was (one group: the second Timed insemination at 16 and 26 hours after GnRH injection, group two: timed insemination at 20 and 30 hours after the second GnRH injection)

[0046] The results are shown in Table 3. The results showed that the follicular cyst rate (0%) in a single artificial insemination 24 hours after the second injection of GnRH was the lowest (p0.05). In addition, the growth rate of follicles was the highest at 0.258mm / day (P>0.05). In summary, a single artificial insemination 24 hours after the second GnRH has the best effect on simultan...

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Abstract

The invention provides a simultaneous estrus-timed insemination treatment method for hybrid buffaloes. The method comprises the following steps that S1, intramuscular injection of gonadotropin releasing hormones is conducted on one or more healthy cows in the estrous cycle; S2, intramuscular injection of sodium cloprostenol is conducted; S3, gonadotropin releasing hormones are injected for the second time, and mifepristone is simultaneously injected to promote ovulation; S4, 24 hours after the injection of mifepristone, artificial insemination is conducted; S5, 5 days after the artificial insemination, intramuscular injection of human chorionic gonadotropin is conducted. By means of the method, the estrous cycle of the buffaloes is effectively controlled, and the simultaneous estrus rate,breeding rate and conception rate of the hybrid buffaloes are increased; the survival rate of early embryos is effectively increased; estrus detection is not needed, timed insemination is achieved, and the operation is convenient.

Description

technical field [0001] The invention belongs to the technical field of breeding buffaloes in animal husbandry, and in particular relates to a treatment method for synchronous estrus-timing insemination of hybrid buffaloes. Background technique [0002] Estrus synchronization technology is one of the important reproductive control technologies in modern animal husbandry production, which is helpful to the further promotion of artificial insemination technology and is also an important part of embryo transfer. The GnRH-PG-GnRH (GPG, Ovsynch) estrus synchronization program has been widely used in livestock breeding, and the estrus synchronization effect is relatively good, but the estrus synchronization effect in buffalo breeding is not ideal, mainly for the following three reasons: (1) ) China's milk buffaloes are mainly the hybrid offspring of local buffaloes and Mediterranean, Moura, and Niri-Lafite buffaloes. Domestic buffaloes belong to swamp type buffaloes (2n=48). Both ...

Claims

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Application Information

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IPC IPC(8): A61D19/02A01K67/02
CPCA01K67/02A61D19/027
Inventor 杨利国滑国华阿地力·阿不来提
Owner HUAZHONG AGRI UNIV
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