Fluorescent quantitative internal reference genes and their primers and applications in different tissues in Changshan, Haizhou

Abstract

The present invention discloses fluorescent quantitative internal reference genes of different tissues in Changshan, Haizhou, primers and applications thereof. The internal reference genes are Actin, RPL, RAN, AP‑2 and HSP70 genes, wherein the gene sequence of the Actin gene is as shown in SEQ ID NO.1 As shown, the gene sequence of the RPL gene is shown in SEQ ID NO.3, the gene sequence of the RAN gene is shown in SEQ ID NO.6, the gene sequence of the AP-2 gene is shown in SEQ ID NO.13, and the gene sequence of the HSP70 gene is shown in SEQ ID NO.13. The gene sequence is shown in SEQ ID NO.14. The present invention designs an internal reference gene combination suitable for different tissues in Changshan, Haizhou, reveals the sequence of the internal reference gene, and designs real-time fluorescent quantitative primers accordingly, which not only solves the current situation that there is no internal reference gene in the existing detection of Changshan, Haizhou, but also The designed real-time fluorescent quantitative primers have strong primer specificity when used for gene expression analysis in Haizhou Changshan tissues, which can greatly improve the detection efficiency of real-time fluorescent quantitative detection of Haizhou Changshan genes, and improve the credibility of the detection results Spend.

Description

technical field [0001] The invention belongs to the field of plant molecular biology, and specifically relates to fluorescent quantitative internal reference genes of different tissues in Changshan, Haizhou, primers and applications thereof. [0002] technical background [0003] Haizhou Changshan (Clerodendrum trichotomum Thunb.), also known as stinky phoenix tree, is a deciduous shrub or small tree under the Verbenaceae (Verbenaceae) Daqing genus (Clerodendrum L.). The leaves of Haizhou Changshan are large and can be used as medicine. Edible wild vegetable, with beautiful flowers and bright fruit, purple-red calyx, 5-lobed almost to the base, long flowering period and huge amount of flowers. The drupe is spherical, blue-purple when ripe, shiny, enclosed in the persistent calyx, and does not fall for a long time in autumn and winter. It is a good fruit-watching tree species in autumn and winter. Haizhou Changshan not only has medicinal functions but also has excellent chara...

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Problems solved by technology

There is no report on the molecular mechanism of Changshan tissue in Haizhou. According to the research, there is no internal reference gene that is constantly expressed under all experimental conditions, and the commonly used internal reference genes will show unstable shapes under different plants and experimental conditions, thus Affects the accuracy of target gene detection, so to develop good genes in Changshan, Haizhou, it is necessary to determine stable and reliable internal reference genes

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