A rapid method for identification of A. gigantea

Abstract

The invention belongs to the technical field of molecular biological identification methods, and in particular relates to a method for rapidly identifying Alysia gigantea, which includes collecting fly stumps and extracting DNA samples, designing specific primers, performing PCR amplification on the samples to be identified, and performing PCR amplification on the samples to be identified. The amplified product was electrophoresed on an agarose gel, and it was judged whether the sample was A. gigantea according to the presence or absence of fragment bands. The present invention designs specific primers for A. gigantea, uses rapid molecular identification SS-PCR technology to quickly and accurately identify it, and can identify larvae, eggs or pupae, and even head, chest, wings, feet, etc. Residue identification greatly overcomes the difficulty of morphological identification and shortens the period of forensic identification.

Description

technical field [0001] The invention belongs to the technical field of molecular biological identification methods, in particular to a method for rapidly identifying Alysia gigantea. Background technique [0002] Sarcophilus flies are the most studied and applied in forensic entomology, and their populations show different growth and development and community succession rules with the change of death time, which is of great significance for the estimation of the postmortem interval (PMI). PMI deduction has always been the focus and difficulty of forensic investigation of criminal cases, and there are more and more cases of using the succession law of necrophagous insects to provide clues for the detection of criminal cases. [0003] There are often larvae, eggs or pupae of different necrophagic flies at the death scene, and even head, thorax, wings, feet and other remnants, which make it difficult to accurately identify the species of necrophagic flies. The classification a...

AI Technical Summary

Problems solved by technology

[0003] There are often larvae, eggs or pupae of different necrophagous flies at the death scene, and even head, chest, wings, feet and other remnants, which makes it difficult to accurately identify the species of necrophagous flies

The classification and identification of larvae is much more difficult than that of adults. Usually, larvae are reared indoors and then identified as adults, which takes a long time, and morphological identification requires rich experience. There are only a few morphological species in my country. Experts have the ability to

In view of the diversity and complexity of flies at the crime scene, it is difficult for non-insect professionals to identify their morphological species, so it is urgent to develop methods for the rapid identification and identification of different sarcophilus flies

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