Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Serum-free medium for culturing mesenchymal stem cells and preparation method of serum-free medium

A serum-free medium and stem cell technology, applied in the field of stem cells, can solve the problems of poor safety and unsuitable application of the medium, and achieve the effect of reducing allergens, avoiding the interference of different concentrations and location components, and being highly targeted.

Inactive Publication Date: 2019-02-26
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
View PDF4 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of this, the main purpose of the present invention is to provide a serum-free medium for culturing mesenchymal stem cells, which is used to solve the problem that the existing medium is poor in safety and not suitable for clinically culturing amniotic mesenchymal stem cells

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Serum-free medium for culturing mesenchymal stem cells and preparation method of serum-free medium
  • Serum-free medium for culturing mesenchymal stem cells and preparation method of serum-free medium
  • Serum-free medium for culturing mesenchymal stem cells and preparation method of serum-free medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] This example provides a serum-free medium for cultivating mesenchymal stem cells, the formula and preparation process of which are as follows:

[0021] 1. Formula

[0022]

[0023]

[0024] 2. Preparation process

[0025] 1) Preparation of stem cell growth factor

[0026] Take human amniotic mesenchymal stem cells at P2, dilute them with low-sugar DMEM containing 10% FBS, and then press 1×10 5 ~10×10 5 The cell density of cell / mL is seeded in culture flasks for large-scale culture.

[0027] When the growth confluence of the cells reaches more than 80%, the cells are washed twice with PBS, and then replaced with 1640 selective medium to culture the cells, and the medium is changed every 3 days, and the culture medium of the cultured cells is collected.

[0028] The culture solution was filtered with a 0.45 μm filter membrane to remove large particles to obtain a primary filtrate. Filter the primary filtrate with a 0.22 μm filter membrane to remove small partic...

Embodiment 2

[0033] This example provides a serum-free medium for cultivating mesenchymal stem cells, the formula and preparation process of which are as follows:

[0034] 1. Formula

[0035]

[0036] 2. Preparation process

[0037] 1) Preparation of stem cell growth factor

[0038] Take human amniotic mesenchymal stem cells at P2, dilute them with low-sugar DMEM containing 10% FBS, and then press 1×10 5 ~10×10 5 The cell density of cell / mL is seeded in culture flasks for large-scale culture.

[0039] When the growth confluence of the cells reaches more than 80%, the cells are washed twice with PBS, and then replaced with 1640 selective medium to culture the cells, and the medium is changed every 3 days, and the culture medium of the cultured cells is collected.

[0040] The culture solution was filtered with a 0.45 μm filter membrane to remove large particles to obtain a primary filtrate. Filter the primary filtrate with a 0.22 μm filter membrane to remove small particles below 3K...

Embodiment 3

[0045] This example provides a serum-free medium for cultivating mesenchymal stem cells, the formula and preparation process of which are as follows:

[0046] 1. Formula

[0047]

[0048] 2. Preparation process

[0049] 1) Preparation of stem cell growth factor

[0050] Take human amniotic mesenchymal stem cells at P2, dilute them with low-sugar DMEM containing 10% FBS, and then press 1×10 5 ~10×10 5 The cell density of cell / mL is seeded in culture flasks for large-scale culture.

[0051] When the growth confluence of the cells reaches more than 80%, the cells are washed twice with PBS, and then replaced with 1640 selective medium to culture the cells, and the medium is changed every 3 days, and the culture medium of the cultured cells is collected.

[0052] The culture solution was filtered with a 0.45 μm filter membrane to remove large particles to obtain a primary filtrate. Filter the primary filtrate with a 0.22 μm filter membrane to remove small particles below 3K...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
percent by volumeaaaaaaaaaa
Login to View More

Abstract

The invention belongs to the technical field of stem cells and in particular relates to a serum-free medium for culturing mesenchymal stem cells and a preparation method of the serum-free medium. Theserum-free medium for culturing the mesenchymal stem cells comprises human albumin, an epidermal growth factor and a stem cell growth factor. The medium is definite in components, and interference ofdifferent concentrations and position components of an inhibitor factor TFG-b of serum can be avoided and allergens can be reduced; by virtue of properties of the stem cell growth factor, the serum-free medium is very good in specificity, and the promotion on proliferation of the mesenchymal stem cells and maintenance of cell dryness of the mesenchymal stem cells is facilitated.

Description

technical field [0001] The invention belongs to the technical field of stem cells, and in particular relates to a serum-free medium for culturing mesenchymal stem cells and a preparation method thereof. Background technique [0002] Mesenchymal stem cells are a type of cells derived from the mesoderm and ectoderm in the early stages of development. They have the characteristics of self-replication, self-renewal, multi-directional differentiation potential, hematopoietic support, and immune regulation. At present, mesenchymal stem cells are mainly extracted from fat, bone marrow, umbilical cord, and placenta tissue. Mesenchymal stem cells still have multi-directional differentiation potential after continuous subculture and cryopreservation, which provides a new way for the clinical treatment of various diseases, such as nervous system diseases, kidney diseases, autoimmune diseases, and malignant tumors. Mesenchymal stem cells derived from human amniotic membrane have the ad...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775
CPCC12N5/0668C12N2500/90C12N2501/11C12N2501/125C12N2501/998
Inventor 王一飞陈海佳葛啸虎梁美乐王小燕
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com