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Buffer solution for liquid-based cytological experiment

A cytology and buffer technology, applied in the buffer field of liquid-based cytology experiments, can solve the problems of poor pH value retention, poor pertinence, and high cost of buffer preparation. The effect of saving time

Inactive Publication Date: 2019-02-26
武汉宏强医疗器械有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the high cost of the existing buffer preparation, and the lack of pertinence for the maintenance of the reaction system during the liquid-based cytology experiment, the maintenance effect on the pH value is not good

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1, the buffer preparation method of the present invention is sodium chloride 1.9%, potassium chloride 1.4%, distilled water 87%, potassium dihydrogen phosphate 6.8%, sodium hydroxide 2.9%, let above-mentioned each component evenly stir and dissolve Can.

[0021] The buffer solution prepared in the above examples reduces the cost of use during use, keeps the reaction system at a constant pH value in liquid-based cytology experiments, and maintains osmotic pressure and ion concentration close to the pH value of the human body (isotonic), Adjust the pH value without affecting the reaction of liquid-based cytology experiments, so that the reaction system of liquid-based cytology experiments can run under the best conditions, easy to promote, highly targeted, and save the time for sample preparation.

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PUM

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Abstract

The invention provides a buffer solution for liquid-based cell tableting. The buffer solution is composed of sodium chloride, potassium chloride, distilled water, potassium dihydrogen phosphate and sodium hydroxide. The buffer solution reduces a use cost, effectively keeps the pH of a reaction system in a liquid-based cytology experiment stable, maintains the osmotic pressure and ion concentrationclose to the human body pH (isotonic) and adjusts the pH value without affecting the liquid-based cytological reaction so that the reaction system of the liquid-based cytology experiment is used under optimal conditions. The buffer solution is easy to promote, has strong specificity and saves the sample preparation time.

Description

technical field [0001] The invention relates to the technical field of biological preparations; in particular, it relates to a buffer solution for liquid-based cytology experiments. Background technique [0002] Phosphate Buffered Saline (PBS) is a buffer solution commonly used in biological research. PBS can be the English abbreviation of three kinds of solutions, which are phosphate buffered solution (phosphate buffered solution), phosphate buffered saline (phosphate buffered saline) and phosphate buffered sodium (phosphate buffered sodium). The biological roles played are not exactly the same. Unless otherwise specified, the commonly used PBS in biology is neutral phosphate buffered solution (phosphate buffered solution). It is a water-based salt solution containing sodium chloride, phosphate, and (in some formulations) potassium chloride and potassium phosphate. Due to the high preparation cost of the existing buffer solution, and the lack of pertinence for maintainin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/00
CPCC12N5/0018C12N2500/12
Inventor 不公告发明人
Owner 武汉宏强医疗器械有限公司
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