Preparation method and device of autologous platelet-rich plasma for treating pressure injury

Abstract

The invention discloses a preparation method and device of autologous platelet-rich plasma for treating pressure injury. The preparation method includes the steps: (1) whole blood collection; (2) anticoagulant adding; (3) primary centrifugation; (4) secondary centrifugation; (5) thrice centrifugation; (6) platelet-rich plasma preparation. The preparation device comprises a centrifugal tube, the centrifugal tube comprises an outer tube, an inner tube and a sealing cover, the inner wall of the outer tube is closely fitted with the outer wall of the inner tube, the sealing cover is hermetically connected with the upper ends of the outer tube and the inner tube, a liquid discharging tube is arranged at the bottom of the inner tube, a simple clamp is arranged on the liquid discharging tube, a plurality of liquid pumping holes are formed in the side wall of the inner tube, side wall sealing parts are arranged in the liquid pumping holes, and needle heads can penetrate the side wall sealing parts. The preparation device is simple in structure and convenient to operate, the pollution probability of blood can be reduced in preparation process of the plasma to a great extent, platelets in the prepared plasma are high in concentration, extracting efficiency is high, preparation period is short, feasibility is high, and the preparation device has popularization and use values in clinical practice.

Description

technical field [0001] The invention belongs to the technical field of platelet-rich plasma extraction, and in particular relates to a preparation method and device for autologous platelet-rich plasma for treating pressure injuries. Background technique [0002] Common pressure injuries include pressure sores and decubitus ulcers, which are caused by long-term pressure on local tissues, malnutrition, and blood circulation disorders, resulting in continuous ischemia and hypoxia of local tissues, which eventually lead to local tissue necrosis. Nursing of pressure injuries is difficult , the treatment is more difficult. Autologous platelet-rich plasma, referred to as PRP, is the plasma containing high concentration of platelets obtained by centrifugation of autologous whole blood, and platelets contain a large number of growth factors, such as platelet-derived growth factor, transforming growth factor, insulin-like growth factor, epidermal growth factor These growth factors ca...

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Problems solved by technology

[0003] The concentration of platelets in human whole blood is generally 1-3×10 5 / ml, studies have shown that the increase in the concentration of concentrated PRP platelets can effectively improve the proliferation and differentiation capabilities of stem cells, and can also significantly increase the proliferation of fibroblasts and the expression of type I collagen. At present, there are manual methods and equipment preparation methods for the extraction of PRP , the existing problems are as follows: one is that the extraction method is more cumbersome, the equipment is expensive and the operation is complicated; the other is that the PRP concentration after extraction is not high, the purity is not good, and it is easily polluted by the outside during the extraction process; the third is that the extraction efficiency is low , these problems make PRP limited in the clinical treatment of pressure injury

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