Fungus detection method based on liquid-based slide making technology and fluorescent staining technology
A fluorescent dyeing and film-making technology, which is applied in the field of fungal detection, can solve the problems of many reagents required for matching, troublesome storage and management, and high professional skills requirements, so as to achieve clear fungal morphology and structure, improve sensitivity and efficiency, and facilitate search and identification Effect
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Embodiment 1
[0067] The "gold standard" fungal culture method:
[0068] 1. Turn on the ultraviolet light of the ultra-clean workbench for disinfection and sterilization half an hour in advance, and clean and disinfect the operator's clothes and hands.
[0069] 2. Take out the prepared culture medium and number it correspondingly to the gynecological sample.
[0070] 3. According to the corresponding number, open the plate and drop a drop of sterilized physiological saline on it, dip the sampling swab in physiological saline and spread it evenly on the plate, cover the petri dish, and put the sampling swab back to its original place. tube.
[0071] 4. Invert the petri dish and incubate in a biochemical incubator at 33°C for 24-72 hours, during which time observe the growth of the colony in the petri dish.
Embodiment 2
[0073] Ordinary fungal fluorescent staining method:
[0074] 1. Rotate the sample evenly to the middle of the slide,
[0075] 2. Add fungal fluorescent staining solution dropwise, cover with a cover glass, and examine under the microscope.
Embodiment 3
[0077] Fungal fluorescent staining method based on liquid-based film production technology
[0078] 1. Collect the sample and put the sample into the sample kit containing the cell preservation solution;
[0079] 2. Place the sample kit on the shaker and shake to mix;
[0080] 3. Install the sample filter and slide glass on the liquid-based fungal film maker;
[0081] 4. Add all the sample liquid in the sample kit to the installed sample filter;
[0082] 5. Start the liquid-based fungal film maker for automatic film production;
[0083] 6. Take out the slide after the production is completed, add 50ul fluorescent staining solution dropwise on the slide, cover it with a cover glass and let it stand for 30s;
[0084] 7. Perform microscopic examination under a fluorescent microscope.
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