A kind of preparation method and application of compound tobacco leaf extract
A technology of tobacco leaf extract and tobacco leaf, which is applied in the field of preparation of compound tobacco leaf extract, to achieve the effects of increased yield, high efficiency, and increased sugar content
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Embodiment 1
[0031] A preparation method of compound tobacco leaf extract, comprising the steps of:
[0032] (1) Raw material pretreatment: the composite tobacco leaves are pulverized into 40-mesh powder by a pulverizer, and the composite tobacco leaves are a mixture of sun-cured red tobacco, sun-cured yellow tobacco and burley tobacco, with a mass ratio of 1:1:2.
[0033] (2) Activation of microbial strains: the active solid freeze-dried bacterial powder (food grade, activity of 10u / mg) of Saccharomycopsis fibuligera and Raoultella planticola VP4-4, The mass concentration that is added to the sterilization treatment of 5 times of mass is in the solution of 5% fructose (food grade), is placed in 35 ℃ of water baths and activated 30min, obtains microbial strain activation solution, wherein said Raoultella planticola (Raoultella planticola ) The VP4-4 collection number is CCTCC M 2012005, which was deposited in the Chinese Type Culture Collection Center of Wuhan University on January 11, 201...
Embodiment 2
[0041] A preparation method of compound tobacco leaf extract, comprising the steps of:
[0042] (1) Raw material pretreatment: the composite tobacco leaves are pulverized into 80-mesh powder by a pulverizer, and the composite tobacco leaves are a mixture of sun-cured red tobacco, sun-cured yellow tobacco and burley tobacco, with a mass ratio of 1:1.2:1.2;
[0043] (2) Activation of microbial strains: the active solid freeze-dried bacterial powder (food grade, activity of 15u / mg) of Saccharomycopsis fibuligera and Raoultella planticola VP4-4, The mass concentration that joins 12 times of mass sterilization treatment is in the solution of 12% glucose (food grade), is placed in 42 ℃ of water baths and activated 45min, obtains microbial strain activation solution, wherein said Raoultella planticola (Raoultella planticola) The VP4-4 collection number is CCTCC M 2012005, and it was deposited in the Chinese Type Culture Collection Center of Wuhan University on January 11, 2012;
[0...
Embodiment 3
[0052] A preparation method of compound tobacco leaf extract, comprising the steps of:
[0053] (1) Pre-treatment of raw materials: the composite tobacco leaves are pulverized into 120-mesh powder by a pulverizer, and the composite tobacco leaves are a mixture of sun-cured red tobacco, sun-cured yellow tobacco and Burley tobacco, with a mass ratio of 1:1:0.5;
[0054] (2) Activation of microbial strains: the active solid freeze-dried bacterial powder (food grade, active 30u / mg) of Saccharomycopsis fibuligera and Raoultella planticola VP4-4 was added to The mass concentration of sterilization treatment to 20 times the mass is 30% glucose (food grade) solution, placed in a 50 ° C water bath for activation for 60 min, to obtain a microbial strain activation solution, wherein the plant Raoultella planticola (Raoultella planticola) The VP4-4 collection number is CCTCC M 2012005, and it was deposited in the Chinese Type Culture Collection Center of Wuhan University on January 11, 2012...
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