Method for breeding rape by near-distant hybridization of cabbage type rape recessive genic male sterile line

Abstract

The invention belongs to the technical field of rape breeding, and discloses a method for breeding rape by a near-distant hybridization of a cabbagetype rape recessive genic male sterile line. The mothod comprises the following steps of: selecting an original parent: selecting a stable recessive genic male sterile two-line material for the cabbage type rape; selecting a material or variety of Brassica, Cruciferae or more distant relationship for the male parent; carrying out the distant hybridization in an initial flowering period of the recessive genic male sterile line by adopting a twice-pollination method; harvesting hybrid seeds in a mature period, and adopting a seedling raising and transplanting method for a hybrid F1; collecting pollen from F2-F3 generation for microspore culture to obtain hybrid progeny strain material. The method for breeding rape by the near-distant bybridization of the cabbage type rape recessive genic male sterile line effectively reduces bud stripping time of the female parent and reduces the damage to a stigma of the female parent; avoids the influence of the female parent pollen on the hybrid progeny; effectively improves the success probability ofthe hybrid and introduces genes of outer edge species into genome of the cabbage type rape.By the adoption of the method for breeding rape by the near-distant bybridization of the cabbage type rape recessive genic male sterile line,new materials of the cabbage type rape such as early maturing, short stalk, white flower and the like are obtained.

Description

technical field [0001] The invention belongs to the technical field of rapeseed breeding, and in particular relates to a method for breeding rapeseed by close and distant hybridization of recessive genic male sterile lines of Brassica napus. Background technique [0002] At present, the existing technologies commonly used in the industry are as follows: [0003] Brassica napus (AACC, 2n=38) is an allotetraploid, derived from diploid cabbage. Brassicarapa (AA, 2n=20) and diploid Brassica oleracea (CC, 2n=18) are formed by natural doubling. Compared with the original species, its evolutionary history is shorter. It was introduced to China about 80 years ago, and its genetic basis is relatively narrow. To improve the yield, quality and resistance potential of Brassica napus varieties, it is urgent to expand the genetic basis of Brassica napus. Breeding Brassica napus varieties suitable for agricultural production and industrial use is the goal of rapeseed breeders. Rapeseed b...

AI Technical Summary

Problems solved by technology

[0008] (1) The hybridization affinity of the prior art is low, and it is difficult to receive true hybrid seeds;

[0009] (2) hybridization efficiency is low in the prior art, and it is easy to produce false hybrids;

[0010] (3) It is difficult to introduce heterologous and heterogeneous favorable genes into Brassica napus in the prior art

[0012] The introduction of heterogeneous and heterogenous beneficial genes into Brassica napus through distant hybridization has always been an important means for rapeseed breeders to broaden the genetic basis and innovate germplasm of Brassica napus. However, it is difficult to receive real hybrid seeds by conventional detasseling methods, even Received a small number of seeds with a high proportion of false hybrids

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