Primer and probe for identification of sturgeon based on quantitative real time polymerase chain reaction (PCR) as well as detection reagent kit and detection method thereof

Abstract

The invention discloses a detection primer and a probe for rapid identification of sturgeon based on quantitative real time polymerase chain reaction (PCR) as well as a detection reagent kit and a detection method which adopt the primer and the probe. A detection primer group comprises a forward primer FP, a backward primer BP and the probe; the detection reagent kit comprises a primer solution, aPCR mix reaction solution, deionized water, a negative control and a positive control; and the detection method comprises the following steps: the DNA of a sample to be examined is extracted; the quantitative PCR is utilized for rapidly identifying the DNA of the sample to be examined; and whether the sample to be examined is the sturgeon or not is judged by a real-time amplification curve. The invention has the advantages of high speed, sensitivity, specificity, easy operation, and the like, and is suitable for popularization and application.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and relates to a primer and a probe for detecting sturgeon based on fluorescence quantitative PCR, a kit and a method thereof. Background technique [0002] Sturgeon (Acipenser), belonging to the class of bony fish, subclass of rayfin, and sturgeon, is an ancient fish with high scientific research, medicinal and ornamental value. Sturgeon is a high-protein, fatty fish, its skin can be made into high-quality special leather, fish eggs can be made into caviar, fish gallbladder can be used as medicine, fish meat, fish intestines and fish bones are all excellent delicacies. It is "the whole body is treasure". However, due to human factors affecting the living environment of sturgeon and overfishing and other factors, the number of wild sturgeon has dropped sharply. In order to seek high profits, many businesses in the processing, market circulation and other links, there are shoddy, indis...

AI Technical Summary

Problems solved by technology

At present, there are few reports on the identification of sturgeon, mainly relying on the traditional morphological identification method, but practitioners need to have strong professional background knowledge, which is greatly affected by subjective judgment and is prone to errors

The detection methods based on molecular biology mainly include microsatellite molecular markers, random primer amplification polymorphism, restriction length polymorphism, etc., but these methods have disadvantages such as time-consuming, complicated operation, poor repeatability, and large errors.

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