Cellobiohydrolase mutant and its production method and application
A technology of cellobiose and hydrolase, which is applied to the composition containing enzyme, the mutant of cellobiohydrolase, and the application field of cellulose degradation, can solve the problem of low efficiency of cellulase hydrolysis and accelerate cellobiohydrolase Problems such as hydrolysis efficiency, to achieve the effect of improving hydrolysis effect, improving hydrolysis efficiency and high activity
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[0029] The present invention will be further described in detail below in conjunction with the accompanying drawings, so that those skilled in the art can implement it with reference to the description.
[0030] It should be understood that terms such as "having", "comprising" and "including" as used herein do not entail the presence or addition of one or more other elements or combinations thereof.
[0031] The present invention provides a cellobiohydrolase mutant, the enzyme is the following (a) or (b) protein:
[0032] (a) Threonine at position 393 of the amino acid sequence shown in SEQ ID NO: 1 is replaced with other amino acids, the other amino acids being lysine, arginine or glutamic acid,
[0033] (b) A protein derived from (a) in which the amino acid sequence in (a) has been substituted, deleted or added by one or several amino acids and has cellobiohydrolase activity; activity of said cellobiohydrolase mutant Provides a degradation function in cellulose degradation....
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