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Mesenchymal stem cell preserving fluid for venous re-transfusion and preparation method of mesenchymal stem cell preserving fluid

A technology of mesenchymal stem cells and preservation solution, which is applied in the field of mesenchymal stem cell preservation solution for intravenous reinfusion and its preparation, can solve the problems such as the reduction of cell viability and biological efficacy, and achieve the improvement of cell utilization rate and prolongation of preservation time. , the effect of reducing the risk of exogenous infection

Inactive Publication Date: 2019-02-15
CHENGDU QINGKE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the above-mentioned deficiencies in the prior art, the present invention provides a mesenchymal stem cell preservation solution for intravenous infusion and a preparation method thereof, which can effectively solve the problem that the existing mesenchymal stem cell preservation solution causes cells to The problem of greatly reduced viability and biological potency

Method used

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  • Mesenchymal stem cell preserving fluid for venous re-transfusion and preparation method of mesenchymal stem cell preserving fluid
  • Mesenchymal stem cell preserving fluid for venous re-transfusion and preparation method of mesenchymal stem cell preserving fluid

Examples

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Effect test

Embodiment 1

[0032] A mesenchymal stem cell preservation solution for intravenous infusion, comprising the following components by weight percentage:

[0033] Autologous plasma lysate mixture 5%, 25% human serum albumin 1%, VC injection 1%, 10% low molecular weight dextran glucose injection 3%, compound electrolyte injection 30%, glucose sodium chloride injection 30% And compound amino acid injection 30%.

[0034] Wherein, the preparation method of autologous plasma lysate mixture is:

[0035] (1) Collect 16 mL of peripheral blood from the donor in EDTA anticoagulant blood collection tubes, and complete the plasma preparation within 2 hours of collection;

[0036] (2) Centrifuge at 4000r / min for 8min, absorb the upper layer of plasma and gently blow the buffy coat to mix the buffy coat and plasma evenly, transfer the plasma and buffy coat to cryopreservation tubes, and temporarily store them in a -80° ultra-low temperature refrigerator stay overnight;

[0037] (3) Resuscitate the plasma...

Embodiment 2

[0043] A mesenchymal stem cell preservation solution for intravenous infusion, comprising the following components by weight percentage:

[0044] Autologous plasma lysate mixture 5%, 25% human serum albumin 5%, VC injection 5%, 10% low molecular weight dextran glucose injection 3%, compound electrolyte injection 27%, glucose sodium chloride injection 28% And compound amino acid injection 27%.

[0045] Wherein, the preparation method of autologous plasma lysate mixture is:

[0046] (1) Collect 16 mL of peripheral blood from the donor in EDTA anticoagulant blood collection tubes, and complete the plasma preparation within 2 hours of collection;

[0047] (2) Centrifuge at 4000r / min for 8min, absorb the upper layer of plasma and gently blow the buffy coat to mix the buffy coat and plasma evenly, transfer the plasma and buffy coat to cryopreservation tubes, and temporarily store them in a -80° ultra-low temperature refrigerator stay overnight;

[0048] (3) Resuscitate the plasma...

Embodiment 3

[0054] A mesenchymal stem cell preservation solution for intravenous infusion, comprising the following components by weight percentage:

[0055] Autologous plasma lysate mixture 1%, 25% human serum albumin 5%, VC injection 1%, 10% low molecular weight dextran glucose injection 3%, compound electrolyte injection 30%, glucose sodium chloride injection 30% And compound amino acid injection 30%.

[0056] Wherein, the preparation method of autologous plasma lysate mixture is:

[0057] (1) Collect 16 mL of peripheral blood from the donor in EDTA anticoagulant blood collection tubes, and complete the plasma preparation within 2 hours of collection;

[0058] (2) Centrifuge at 4000r / min for 8min, absorb the upper layer of plasma and gently blow the buffy coat to mix the buffy coat and plasma evenly, transfer the plasma and buffy coat to cryopreservation tubes, and temporarily store them in a -80° ultra-low temperature refrigerator stay overnight;

[0059] (3) Resuscitate the plasma...

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Abstract

The invention discloses a mesenchymal stem cell preserving fluid for venous re-transfusion and a preparation method of the mesenchymal stem cell preserving fluid. The preserving fluid comprises the following components in percentage by weight: 1-5% of an autologous plasma lysate mixed liquid, 1-5% of human albumin, 1-5% of a VC (Vitamin C) injection, 2-3% of a glucose injection, 23-30% of a compound electrolyte injection, 23-30% of a glucose salt injection and 23-30% of a compound amino acid injection. The preserving fluid disclosed by the invention is capable of achieving direct re-transfusion after temporary preservation of refrigeration, cell injury of cells preserved at a frozen state in a preparation process and a cell re-treatment process after anabiosis can be reduced, and the cellutilization rate can be increased.

Description

technical field [0001] The invention belongs to the technical field of cell preservation, and in particular relates to a mesenchymal stem cell preservation solution for intravenous infusion and a preparation method thereof. Background technique [0002] Stem cells can be used to treat and repair a variety of damaged cells or tissues based on their homing, migration, proliferation and differentiation characteristics. At the same time, stem cells can secrete a variety of cytokines through paracrine action, such as growth factors, chemokines, etc. Cytokines can combine with damaged cell receptors to achieve the repair of damaged cells. [0003] Cell therapy involves processes such as cell preparation, expansion, and reinfusion. Usually, the place and time of these processes are not consistent, and the quality control of cells during transportation is weak. In the normal temperature environment of the cell, various enzymes in the cell react with other biological macromolecules,...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226
Inventor 高雪华海泉陈静娴赵峻
Owner CHENGDU QINGKE BIOTECH
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