Refrigeration device for collecting microbial samples in water, device and method for collecting microbial samples in water

Abstract

The invention discloses a refrigeration device for collecting microbial samples in water, a device and a method for collecting microbial samples in water. The enrichment device includes a refrigerated shell and an enrichment collection device coaxially arranged in the refrigerated shell; the refrigerated shell includes two opposite half shells, the middle of the refrigerated shell is provided with a water pipe perforation, and the water pipe A cooling device is arranged between the perforation and the inner wall of the shell; the ring clip of the enrichment collection device; and a filter membrane and a sand core filter are sandwiched between the ring clip. The collection device includes: a water inlet pipe, a water outlet pipe, and at least one enrichment pipeline connected in parallel between the water inlet pipe and the water outlet pipe; the enrichment pipeline is composed of a first valve, an enrichment collection device and a flow meter connected in series through pipelines composition. The method includes the following steps: using the filter membrane to collect microorganisms in the water in real time, and calculating the flow rate and flow rate in real time during the collection process; and cooling the filter membrane in real time during the collection process, and the cooling temperature is 3-5 degrees.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, in particular to a method for collecting and pretreating microorganism samples. Background technique [0002] Due to the widespread use and abuse of antibiotics, the residual antibiotics in the environment exert selective pressure on the microbial community, induce the production of antibiotic resistance genes (ARGs), persist in the environment and spread widely, and have a negative impact on the ecological environment and human beings. Health comes with huge risks. At present, ARGs have become a research hotspot in academia. Pharmaceutical wastewater often contains high concentrations of antibiotics and production raw materials and intermediates, which form a high-intensity selective pressure on the microbial community of the biological treatment unit, providing favorable conditions for the persistent existence and spread of ARGs in sewage. Pharmaceutical wastewater is often discharged ...

AI Technical Summary

Problems solved by technology

The sample collection and pretreatment process is cumbersome and time-consuming, with high requirements for transportation and storage, and the risk of external source contamination is high

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