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Exposure device used for researching chronic toxicity of polluted sediments for fishes

A chronic toxicity and sediment technology, applied in the direction of measuring devices, test sample preparation, sampling, etc., can solve the problems of fish risk thresholds to be established, different fish exposure routes and mechanism of action are not clear, to avoid exposure Test for heterogeneous biological responses, ensure homogeneity, and improve representativeness

Pending Publication Date: 2019-02-05
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the risk threshold of pollutants in sediments to fish has yet to be established, and the exposure route and mechanism of action to different fish are still unclear, and relevant research is urgently needed

Method used

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  • Exposure device used for researching chronic toxicity of polluted sediments for fishes
  • Exposure device used for researching chronic toxicity of polluted sediments for fishes
  • Exposure device used for researching chronic toxicity of polluted sediments for fishes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] A schematic diagram of an exposure device for studying the chronic toxicity of polluted sediments to fish according to the present invention is as follows figure 1 As shown, it includes an exposure container 1 and a sediment pre-balance container 2. The exposure container 1 is divided into upper and lower parts. The upper part is a test organism exposure chamber 15, and the lower part is a sediment stabilization chamber 7. Partition plate 12 separates, sediment pre-balance container 2 is connected with the hollow conduit 9 in the center of sediment stabilization chamber 7 by peristaltic pump 5 and conveying pipe 6, and sediment obtains the muddy water after balancing by sediment pre-balance container 2, and peristaltic pump 5. Start to transport the balanced mud-water mixture to the sediment stabilization chamber 7 at the lower part of the exposure container 1 through the delivery pipe 6, and the mud-water mixture transported from the pre-balance container 2 is dispersed...

Embodiment 2

[0045] Such as figure 1 As shown, an exposure device used to study the chronic toxicity of contaminated sediments to fish, firstly determine the equilibration time of sediments and target pollutants in the pre-equilibrium container 2, which can be obtained by consulting the target pollutants in similar properties The adsorption kinetics in the sediment can be determined from the literature, and it can also be determined by the adsorption equilibrium pre-experiment. The specific steps of the adsorption equilibrium pre-experiment are as follows: From the literature, it can be known that the lowest visible effect concentration LOEC of TB on Japanese medaka and black-headed minnow is 365 ng / L and 401 ng / L respectively, and the solubility of TB in water is 58.6 mg / L , choose 1 to 3 concentrations between 365ng / L and 58.6mg / L (can be increased exponentially), such as 500ng / L, 5μg / L and 50μg / L, and prepare an aqueous solution of the corresponding concentration; determine the depositi...

Embodiment 3

[0048] Utilize the experimental device of embodiment 1 to carry out the indirect exposure of polluted sediment to fish, and the glass fiber filter membrane that the aperture is 0.45 μ m is fixed in the hole 13 with ring splint 14, to ensure that sediment particles are effectively intercepted and dissolved state Contaminants diffuse freely from the subject organism exposure chamber 15 to the top. Since the sediment is intercepted by the filter membrane in the sediment stabilization chamber 7, the mud discharge pipe needs to be opened regularly to discharge the mud, and the amount of mud discharged is about 144g / d. All the other steps and conditions are the same as in Example 2. By measurement, in the present embodiment, the concentration of TB in the exposure chamber 15 is 408ng / L, which is basically consistent with the water phase concentration of TB in equilibrium in Example 2, and no sediment particles are detected in the exposure chamber (concentration is 0mg / L).

[0049...

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Abstract

The invention discloses an exposure device used for researching the chronic toxicity of polluted sediments for fishes, and belongs to the field of aquatic organism toxicity detection. The device mainly comprises an exposure container and a sediment pre-balancing container, wherein sediments are fully mixed with a target pollutant in the pre-balancing container to achieve adsorption balance, then,the mixture is input into a sediment stabilization chamber on the lower part of the exposure container, and is evenly dispersed through a rotary hollow conduit, and then, the mixture enters a tested organism exposure chamber on the upper part of the exposure container along with a bottom-up hydraulic function. The sediment stabilization chamber and the exposure chamber are separated through a waterproof clapboard, the clapboard can be provided with glass fiber filter screens or filter membranes of different apertures through an annular clamping plate so as to control the form of pollutants which enter the tested organism exposure chamber, and the adsorption states of particular matters of different particle sizes under a dissolved state can be distinguished. A turbidimeter or a suspended particulate matter measurement probe is installed on a lantern ring capable of vertically moving on the inner wall of the exposure chamber so as to be convenient in monitoring the concentration of particulate matters of different depths in the exposure chamber in real time.

Description

technical field [0001] The invention belongs to the field of toxicity detection of aquatic organisms, in particular to an exposure device for chronic toxicity of polluted sediments to fish. Background technique [0002] Most of the past toxicology studies assumed that "dissolved pollutants can be used by organisms". Therefore, traditional exposure experiments mostly use water as the medium, and seldom involve other phases or other environmental media. The current various environmental standards also use the water phase concentration of pollutants, and the risk threshold of the solid phase concentration of pollutants has not yet been established. However, the sediment in the water body is an important sink of various pollutants, and the pollutants in the sediment may be released back to the water phase due to changes in environmental conditions (such as water temperature, pH or physical disturbance, etc.). In recent years, studies on new pollutants have shown that the pollut...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/00G01N1/38
CPCG01N1/38G01N33/00G01N2001/386
Inventor 张云罗鑫邹华朱荣丁剑楠付善飞
Owner JIANGNAN UNIV
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