A lrfft1 cell

Abstract

CtDNA sequencing of human-derived peripheral blood or whole exon sequencing of tumor tissues is carried out to screen a mutation site for antigen epitope prediction, and the mutation site undergoes antigen epitope prediction to ligate and synthesize a mutant polypeptide expression gene sequence; construction of a lentiviral vector and packaging of the lentivirus are simultaneously carried out, anAPC cell is transferred, the transformation of a specific LV cell is completed, an in vitro PBMC and a PBMC isolated from peripheral blood are co-cultured, an effective peptide is screened, and an ordinary T cell is transformed through accurate and effective peptide stimulated secondary impact to form an RFF cell having an accurate killing capability; transformation is carried out by using a TCR-Ttechnology principle; and the modified T cell undergoes immunosuppressive target knockout by using a gene editing technology, so a specific killer T cell is accurately protected from being inhibitedin vivo, the lethality of the T cell on tumor cells is improved, and the offensive and defensive LRFFT1 cell is obtained.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a LRFFT1 cell and a preparation method thereof. Background technique [0002] At present, in terms of tumor-specific immunotherapy, the existing LAK, DC, CIK, and DC-CIK cells and methods have basically been proved to be ineffective, while NK, CAR-NK, TIL and other cell technologies have yet to mature. CAR- T cells also have shortcomings in safety and solid tumor therapy. In the prior art, DCs are generally modified to present T cells to produce specific killing. Some laboratories are trying to use viruses as vectors to transfect and present T cells to induce specific killing of T cells. We have also used mutant hybrid peptides to directly stimulate PBMCs to induce T cells. There are also laboratories using TCR-T technology to target the presentation of MAGE A3 antigen. [0003] The above treatment methods are immature, especially the in vitro induction of DC cells an...

AI Technical Summary

Problems solved by technology

There is a general lack of accurate and effective analysis of patient antigens

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