Method for field doubling propagation of healthy sugarcane seedlings
A technology of sugarcane and health, applied in the direction of sugarcane cultivation, horticultural methods, botany equipment and methods, etc., can solve the problems of long production cycle, slow breeding speed, high transportation cost, etc., and achieve reduced transportation costs, fast rooting, and survival rate high effect
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Embodiment 1
[0031] A method for multiplying sugarcane healthy seedlings in the field, comprising the following steps:
[0032] (1) In early and mid-March of the first year, take the stem tips of sugarcane as explants, conduct induction cultivation and multiplication cultivation after disinfection to obtain sugarcane cluster buds.
[0033] (2) Transfer the obtained clustered buds into a culture bag with liquid medium and seal them for rooting cultivation. Liquid medium: MS+sucrose 50 g / L+ naphthalene acetic acid 5 mg / L; pack and seal the culture bag and clustered buds Transport from the tissue culture laboratory to the planting base, place the cultivation bag in a well-ventilated room without direct sunlight, close the doors and windows, and turn on the lights at night.
[0034] (3) Cultivate for 4 days for rooting. After all the roots of clustered buds grow out, open the opening of the cultivation bag, and immediately sterilize the rooted seedlings. After continuing to cultivate for 1 day, ...
Embodiment 2
[0045] A method for multiplying sugarcane healthy seedlings in the field, comprising the following steps:
[0046] (1) In early and mid-March of the first year, take the stem tips of sugarcane as explants, conduct induction cultivation and multiplication cultivation after disinfection to obtain sugarcane cluster buds.
[0047] (2) Transfer the obtained clustered buds into a culture bag with liquid medium and seal them for rooting cultivation. Liquid medium: MS+sucrose 50 g / L+ naphthalene acetic acid 5 mg / L; pack and seal the culture bag and clustered buds Transport from the tissue culture laboratory to the planting base, place the cultivation bag in a well-ventilated room without direct sunlight, close the doors and windows, and turn on the lights at night.
[0048] (3) Cultivate roots for 3 days. After all the roots of clustered buds grow out, open the opening of the cultivation bag, and immediately sterilize the rooted seedlings. After continuing to cultivate for 1 day, they...
Embodiment 3
[0059] A method for multiplying sugarcane healthy seedlings in the field, comprising the following steps:
[0060] (1) In early and mid-March of the first year, take the stem tips of sugarcane as explants, conduct induction cultivation and multiplication cultivation after disinfection to obtain sugarcane cluster buds.
[0061] (2) Transfer the obtained clustered buds into a culture bag with liquid medium and seal them for rooting cultivation. Liquid medium: MS+sucrose 50 g / L+ naphthalene acetic acid 5 mg / L; pack and seal the culture bag and clustered buds Transport from the tissue culture laboratory to the planting base, place the cultivation bag in a well-ventilated room without direct sunlight, close the doors and windows, and turn on the lights at night.
[0062] (3) Cultivate the roots for 5 days. After all the roots of the clustered buds grow out, open the opening of the cultivation bag, and immediately sterilize the rooted seedlings. After continuing to cultivate for 1 d...
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