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ELISA kit for detecting Brucella antibody

The technology of a brucella and a kit is applied in the field of ELISA kits for detecting brucella antibodies, which can solve the problems of many required reagents, long detection period, complicated procedures, etc., and achieves low detection cost and is suitable for promotion. , the effect of high sensitivity

Inactive Publication Date: 2019-01-29
ANIMAL & PLANT & FOOD INSPECTION CENT OF TIANJIN ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Abstract
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  • Claims
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AI Technical Summary

Benefits of technology

This patented technology can detect small amounts of bacteria called Bletilla that causes symptoms such as fever or rheumatoid arthritis (RA). Its technical effect would be improved accuracy and reduced costs compared with current diagnostic techniques used by medical professionals.

Problems solved by technology

This patented technical problem addressed in this patent relates to improving diagnosis techniques based upon molecular biology technologies like PCR and Western blotting. These conventional approaches require expensive equipment, complex operations, and multiple steps involving different chemicals. Therefore there has developed an improved approach towards these new ways overcoming limitations associated with current testing protocols.

Method used

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  • ELISA kit for detecting Brucella antibody
  • ELISA kit for detecting Brucella antibody
  • ELISA kit for detecting Brucella antibody

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Embodiment Construction

[0010] Below in conjunction with specific embodiment the present invention is described in further detail:

[0011] The ELISA kit for detecting the Brucella antibody of the present invention comprises an Omp31 recombinant protein coated plate, and the Omp31 recombinant protein uses a part of the Omp31 gene sequence and obtains it as shown in SEQ ID NO.1 in the sequence listing after codon optimization The nucleotide sequence is shown as the whole gene synthesis. After enzyme digestion, the Omp 31 gene is connected to the pET-28a(+) plasmid. After identification, it is transformed into E. coli BL21 competent cells, induced to express, and obtained by separation and purification by affinity chromatography. of.

[0012] The coating method of the protein-coated plate is as follows: the Omp31 recombinant protein is diluted with 0.5M carbonate buffer, 0.1 mL is added to each well of the 96-well microtiter plate, and coated for 15 hours at 2-8° C.; each well is added with 250 μL Blo...

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Abstract

The invention discloses an ELISA kit for detecting a Brucella antibody. Engineering bacteria is recombined by Omp31 gene construction, expression Omp31 recombination protein is used as a coating antigen to fabricate protein coating plate, and an ELISA antibody detection kit is built. The kit has the advantages of good specificity, high sensitivity and low detection cost and is suitable for promotion. An important method is provided for aspects of antibody level detection of disease, epidemiological investigation, disease identification and effective prevention measurement taking. The kit mainly comprises an Omp31 protein coating plate, positive control serum, negative control serum, an enzyme-labeled rabbit-anti polyclonal antibody, a substrate liquid, a stop liquid, a diluent liquid, a washing liquid and a serum attenuation plate.

Description

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Claims

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Application Information

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Owner ANIMAL & PLANT & FOOD INSPECTION CENT OF TIANJIN ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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