Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Symbiotic drought-resistance seed germination-promotion endophytic bacterium and application thereof

A seed, germination rate technology, applied in application, seed and rhizome treatment, bacteria, etc., can solve problems such as less impact research and less attention to desert plants

Active Publication Date: 2019-01-25
XINJIANG NORMAL UNIVERSITY
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005]At present, most of the researches on plant seed germination are stresses of abiotic factors such as drought, salt, temperature, etc., while there are few studies on the influence of biotic factors, and most of them are symbiotic The research on microbial action focuses on the production of effective metabolic components of medicinal plants, and less attention is paid to desert plants

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Symbiotic drought-resistance seed germination-promotion endophytic bacterium and application thereof
  • Symbiotic drought-resistance seed germination-promotion endophytic bacterium and application thereof
  • Symbiotic drought-resistance seed germination-promotion endophytic bacterium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1, the acquisition of bacillus AER314-4

[0045] 1. Isolation of strains

[0046] 37 strains of endophytic bacteria were isolated from the roots of Sophora chinensis.

[0047] 2. Screening of highly effective strains of endophytic bacteria for drought resistance and seed germination

[0048] Strains to be tested: 37 strains of endophytic bacteria isolated in step one.

[0049] 1. Pick a single colony of the strain to be tested, inoculate it in beef extract peptone liquid medium and cultivate it to the OD of the bacterial solution 600nm to around 1.0.

[0050] 2. Configure and prepare the culture medium as follows:

[0051] Experimental group: Spread double-layer sterile filter paper on a sterilized petri dish, mix 1.4mL of the bacterial solution obtained in step 1 and 2.8mL of 25% PEG6000 solution, and then evenly add to the filter paper;

[0052] Control group A: Spread double-layer sterile filter paper on a sterilized petri dish, mix 1.4 mL of beef ext...

Embodiment 2

[0082] Embodiment 2, the effect of Bacillus sp. AER314-4 on the substance metabolism in the process of seed germination

[0083] 1. The effect of Bacillus sp. AER314-4 on the activity of α-amylase in seeds during the germination process of Sophora chinensis

[0084] 1. Pick a single colony of the strain to be tested, inoculate it in beef extract peptone liquid medium and cultivate it to the OD of the bacterial solution 600nm When it reaches about 1.0, centrifuge the bacterial solution at 4°C and 8000r / min for 10min, collect the bacterial pellet and supernatant respectively, and resuspend the bacterial pellet with an equal volume of sterilized PBS buffer (pH=6.8) for later use.

[0085] 2. Soak the surface-sterilized Sophora japonica seeds in 98% concentrated sulfuric acid for 90 minutes (to rupture the seed coat), and then rinse with a large amount of sterile water until pH=7. Spread the treated seeds evenly in each group of medium in step 2 (25 grains per dish), weigh and re...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a symbiotic drought-resistance seed germination-promotion endophytic bacterium and an application thereof. The invention protects Bacillus mojavensis AER314-4, its deposit number is: CCTCC NO:M2018495. It was confirmed by experiments that Bacillus AER314-4 could increase the germination rate of Sophora japonica seeds by 54% under 25% PEG 6000 drought stress, and the starchwas degraded to produce soluble sugar by activating the amylase in the seeds, which decreased the osmotic potential of the seeds and promoted the germination of water absorption. Bacillus has the characteristics of fast growth, strong resistance, no time and climate limitations, safety for human and animals, no pollution of the environment, no adverse effects on plants. The strain has the potential to be used as desert plant seeds to promote germination under drought stress, and can be applied to desert plant seeds to promote seed germination under drought stress to promote ecosystem restoration.

Description

technical field [0001] The invention relates to a symbiotic drought-resistant endophyte for promoting seed germination and application thereof. Background technique [0002] In order to better adapt to the environment and absorb nutrients, desert plants form a symbiotic relationship with microorganisms. The relatively stable environment in plant tissues is conducive to the survival of microorganisms and the continuation of the population, and the host plant obtains the adaptation function to the adversity from the metabolites of microorganisms or the regulation of genes. benefit from adversity. [0003] Ammodendron bifolium (Pall·) is a sand-growing deciduous shrub of the genus Ammodendron of the family Leguminosae. National Class II List of Endangered Plants. The study found that one of the endangering factors of Sophora chinensis is the difficulty of seed germination under drought conditions. As a native species in Xinjiang, Saccharia chinensis is of great value to the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20A01C1/00C12R1/07
CPCA01C1/00C12R2001/07C12N1/205
Inventor 安登第李艳茹朱智楠时晓寒
Owner XINJIANG NORMAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com