Biological reaction device provided with microfluidic or nanofluidic structure

Abstract

The present application relates to a biological reaction device provided with a microfluidic or nano-fluidic structure, characterized in that the biological reaction device comprises a biological sample extraction bin, a reaction bin, and a microfluidic or nano-fluidic structure for transferring a biological sample from the biological sample extraction bin to the reaction bin, and a microfluidic or nano-fluidic structure for transferring the biological sample from the biological sample extraction bin to the reaction bin. Wherein the microfluidic or nano-fluidic structure comprises a biologicalsample channel and a reaction liquid injection channel, wherein the biological sample extraction bin and the reaction bin are fluidly communicated through the biological sample channel, and the reaction liquid is injected into the reaction bin through the reaction liquid injection channel; wherein the microfluidic or nanofluidic structure further comprises a first valve for blocking or opening the biological sample passage and a second valve for blocking or opening the reaction liquid injection passage. The microfluidic or nanofluidic structure further comprises a first valve for blocking oropening the biological sample passage and a second valve for blocking or opening the reaction liquid injection passage. The biological reaction apparatus of the present invention enables accurate quantification of biological samples and sufficient and efficient transfer to the reaction chamber, simplifies the experimental process, improves the experimental efficiency and reduces the likelihood ofcontamination during the transfer of biological samples.

Description

technical field [0001] The present application relates to the technical fields of biochemistry and molecular biology, and in particular to a bioreaction device provided with a microfluidic or nanofluidic structure. Background technique [0002] With the transformation of the medical model and the continuous development of individualized medicine, the medical testing community urgently needs fast and accurate detection methods, and molecular detection plays a unique role. The basis of molecular detection is to analyze the genes and their expression products in the tissue cells, hair, anticoagulant blood or dried blood stains of the subject, as well as formaldehyde-fixed and paraffin-embedded tissues. ) detection can be accurately detected once the disease occurs or even before the symptoms, signs and biochemical changes appear. [0003] At present, molecular detection technologies mainly include nucleic acid molecular hybridization, polymerase chain reaction (PCR) and biochi...

AI Technical Summary

Problems solved by technology

At present, blood routine, cytology, pathology and immunology and other testing methods are all developing in the direction of automation, integration and standardization, but due to the technical complexity of molecular testing, the fully automated instrument platform "from sample to result" Few or many technical problems that are difficult to solve

For example, the spin column method or magnetic bead method used in the field of biotechnology for nucleic acid extraction generally requires four steps of lysis, binding, rinsing, and elution, plus subsequent nucleic acid molecule hybridization, polymerase chain reaction (PCR ) and biochips and other detection steps, it is very difficult to realize the fully automated instrument "from sample to result". It is obvious that the manual material transfer method used in the prior art is not only cumbersome to operate, but also time-consuming and labor-intensive.

What's more, the whole operation process is easy to cause pollution, which affects the purity of the extract, and it is difficult to transfer the material fully and efficiently, which affects the experimental results.

Therefore, in view of the technical complexity of molecular detection itself, there are few fully automated instrument platforms for "from sample to result" or there are many difficult technical problems. In order to avoid problems in the prior art such as high cost, poor sensitivity, complex structure, overly complicated operation and related detection equipment, etc.

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