Preparation method of formamide phenazine bio-fungicide
A biological fungicide, the technology of caramidoxazine, which is applied in the field of microorganisms, can solve the problems of low yield of carboxyzine, complex fermentation operation, high overall cost, etc., and achieve the effect of good effect, short fermentation cycle and low cost
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[0038] The present invention provides the preparation method of formamide phenazine biological fungicide, comprising the following steps:
[0039] S1. Inoculate the activated Pseudomonas aeruginosa into the liquid medium A and culture it in a shaker flask to obtain the seed liquid A. Wherein, the liquid medium A comprises the following components by weight: 1-5 parts of glucose, 1-5 parts of tryptone, 0.5-1.5 parts of ethanol, 0.5-1 part of acetone, 0.05-0.15 parts of potassium dihydrogen phosphate and chloride 0.05-0.1 parts of sodium, 100-120 parts of water, pH6.5-7.3. The condition of the shake flask culture is: shake culture in a shaker at 25-30° C. for 15-20 hours, and the shaker speed is 200-250 r / min.
[0040] S2. Inoculate the activated Pseudomonas aeruginosa into the liquid medium B, and culture it in a shake flask to obtain the seed liquid B. Wherein, the liquid medium B includes the following components by weight: 0.5-3 parts of sucrose, 0.5-2 parts of glucose, 2-...
Embodiment 1
[0049] A preparation method of formamide phenazine biological bactericide, comprising the following steps:
[0050] S1. Inoculate the activated Pseudomonas aeruginosa into the liquid medium A and culture it in a shaker flask to obtain the seed liquid A. Wherein, the liquid medium A comprises the following components by weight: 3 parts of glucose, 3 parts of tryptone, 1 part of ethanol, 0.6 part of acetone, 0.1 part of potassium dihydrogen phosphate and 0.07 part of sodium chloride, 110 parts of water, pH7. 0. The condition of the shake flask culture is as follows: shaking culture in a shaker at 27° C. for 18 hours, and the speed of the shaker is 220 r / min.
[0051] S2. Inoculate the activated Pseudomonas aeruginosa into the liquid medium B, and culture it in a shake flask to obtain the seed liquid B. Wherein, the liquid medium B comprises the following components by weight: 2 parts of sucrose, 1 part of glucose, 4 parts of tryptone, 1.7 parts of glycerol, 0.1 part of potassi...
Embodiment 2
[0060] A preparation method of formamide phenazine biological bactericide, comprising the following steps:
[0061] S1. Inoculate the activated Pseudomonas aeruginosa into the liquid medium A and culture it in a shaker flask to obtain the seed liquid A. Wherein, the liquid medium A comprises the following components by weight: 1 part of glucose, 1 part of tryptone, 0.5 part of ethanol, 0.5 part of acetone, 0.05 part of potassium dihydrogen phosphate and 0.05 part of sodium chloride, 100 parts of water, pH6. 5. The condition of the shake flask culture is as follows: shaking culture in a shaker at 25° C. for 20 hours, and the speed of the shaker is 200 r / min.
[0062] S2. Inoculate the activated Pseudomonas aeruginosa into the liquid medium B, and culture it in a shake flask to obtain the seed liquid B. Wherein, the liquid medium B comprises the following components by weight: 0.5 part of sucrose, 0.5 part of glucose, 2 parts of tryptone, 1.5 part of glycerol, 0.05 part of pot...
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